PCR, RT-PCR, qPCR and PCR Kits

von Mariem BEN CHOIYA
PCR • RT-PCR • qPCR • Molecular Biology Reagents

PCR Kits, RT-PCR Reagents and qPCR Master Mixes for Reliable DNA and RNA Analysis

Explore Affigen PCR product categories for polymerase chain reaction, RT-PCR, direct PCR, high-fidelity PCR, rapid PCR, qPCR, RT-qPCR, miRNA analysis, host cell residual DNA detection and single-cell sequence amplification.

Explore PCR Categories Choose the Right PCR Kit
Molecular workflow guide

PCR Workflow Focus

From DNA amplification to RNA reverse transcription and real-time qPCR detection, Affigen PCR reagents support reliable molecular biology workflows.

PCR DNA amplification for endpoint molecular biology workflows
RT-PCR RNA to cDNA conversion followed by PCR amplification
qPCR Real-time quantitative detection of DNA or cDNA targets
Choose by workflow: conventional PCR, RT-PCR, direct PCR, high-fidelity PCR, rapid PCR, qPCR master mix, one-step RT-qPCR and miRNA qPCR.

Top PCR Keywords Covered in This Guide

This PCR guide is structured for Google indexing, AI search visibility, GEO optimization and product-category discovery.

PCR RT PCR PCR kit PCR test qPCR Polymerase chain reaction Reverse transcription polymerase chain reaction PCR reagents RT PCR kit qPCR master mix DNA Primer Reaction Temperature Rapid PCR High fidelity PCR miRNA qPCR

Product Category Navigation

Conventional PCR RT-PCR Kit / Transcriptase Direct PCR High-Fidelity PCR Host Cell Residual DNA Single-Cell Amplification miRNA qPCR miRNA Reverse Transcription One-Step RT-qPCR qPCR Master Mix Probe Rapid PCR RT-qPCR SuperMix

What Is PCR and Why Are PCR Kits Important?

PCR, or polymerase chain reaction, is a core molecular biology method used to amplify specific DNA sequences. A PCR kit provides optimized reagents that help researchers perform reliable DNA amplification, RT-PCR, qPCR and RT-qPCR workflows.

Workflow Starting Material Main Purpose Recommended Affigen Category
PCR DNA Endpoint DNA amplification Conventional PCR, Rapid PCR, High-Fidelity PCR
RT-PCR RNA RNA to cDNA followed by amplification Conventional RT-PCR Kit or Transcriptase
qPCR DNA or cDNA Real-time quantitative detection qPCR Master Mix Probe
RT-qPCR RNA RNA target quantification One-Step RT-qPCR Mix, RT-qPCR SuperMix
miRNA qPCR microRNA Small RNA expression analysis miRNA Reverse Transcription, miRNA qPCR

Affigen PCR Product Categories

Discover PCR kits, RT-PCR kits, qPCR reagents and specialized molecular biology products by workflow.

Conventional PCR Kits

Conventional PCR kits are used for standard polymerase chain reaction workflows, endpoint DNA amplification, genotyping, cloning support and target verification.

PCR kit DNA amplification PCR reagents
PCR
AffiPCR™ Taq PCR Master Mix Routine DNA amplification and endpoint PCR workflow
DNA
AffiPCR™ 2X PCR Ready Mix Ready-to-use PCR reagent format for laboratory research
View Conventional PCR

RT-PCR Kit or Reverse Transcriptase

RT-PCR converts RNA into cDNA before PCR amplification. It is used for transcript detection, RNA research and reverse transcription polymerase chain reaction workflows.

RT PCR Reverse transcriptase cDNA synthesis
RT
AffiRT™ Reverse Transcriptase RNA to cDNA conversion for RT-PCR applications
RNA
AffiRT-PCR™ cDNA Synthesis Kit Reverse transcription reagent system for RNA analysis
View RT-PCR Products

Direct PCR Kits

Direct PCR kits simplify sample preparation by allowing DNA amplification from minimally processed samples.

Direct PCR Fast screening Sample-to-PCR
DIR
AffiDirect™ PCR Kit Direct amplification from crude or minimally prepared samples
COL
AffiDirect™ Colony PCR Mix Rapid colony PCR and screening workflows
View Direct PCR

High-Fidelity PCR

High-fidelity PCR reagents are designed for accurate DNA amplification where sequence reliability is important.

High fidelity PCR Proofreading polymerase Accurate amplification
HF
AffiProof™ High-Fidelity DNA Polymerase Proofreading polymerase for accurate PCR amplification
SEQ
AffiProof™ High-Fidelity PCR Master Mix For cloning, sequencing preparation and long amplicons
View High-Fidelity PCR

Host Cell Residual DNA Quantitative Detection

Host cell residual DNA detection kits support quantitative DNA analysis in bioprocessing, QC research and analytical development workflows.

Residual DNA qPCR detection Bioprocess QC
HCD
AffiQuant™ Host Cell DNA qPCR Kit Quantitative detection of host cell residual DNA
QC
AffiQuant™ Residual DNA Detection Mix For process development and analytical research workflows
View Residual DNA Detection

Single-Cell Sequence Amplification

Single-cell sequence amplification reagents are designed for low-input DNA or RNA workflows where limited starting material requires sensitive amplification.

Single-cell PCR Low input Sequence amplification
SC
AffiCellAmp™ Single-Cell Amplification Kit Low-input amplification for single-cell molecular research
NGS
AffiCellAmp™ Sequence Prep Mix Amplification support for downstream sequencing workflows
View Single-Cell Amplification

miRNA qPCR

miRNA qPCR reagents support sensitive microRNA quantification for expression analysis, biomarker research and gene regulation studies.

miRNA qPCR MicroRNA Expression analysis
miR
AffiMIR™ miRNA qPCR Master Mix qPCR reagent system for microRNA quantification
qP
AffiMIR™ MicroRNA Detection Mix For small RNA expression research workflows
View miRNA qPCR

miRNA Reverse Transcription

miRNA reverse transcription products convert small RNA into cDNA before qPCR analysis.

miRNA RT Small RNA cDNA preparation
RT
AffiMIR™ miRNA Reverse Transcription Kit cDNA synthesis for microRNA qPCR workflows
cD
AffiMIR™ Small RNA cDNA Mix Reverse transcription reagent for small RNA analysis
View miRNA Reverse Transcription

One-Step RT-qPCR Mix

One-step RT-qPCR mix combines reverse transcription and quantitative PCR in a single reaction.

One-step RT-qPCR RNA quantification qPCR mix
1S
AffiOneStep™ RT-qPCR Mix Single-tube reverse transcription and qPCR workflow
RNA
AffiOneStep™ RNA Detection SuperMix For RNA target detection and real-time amplification
View One-Step RT-qPCR

qPCR Master Mix Probe

qPCR probe master mixes enable real-time quantitative PCR using sequence-specific fluorescent probes.

qPCR Probe master mix Real-time PCR
qPCR
AffiQ™ Probe qPCR Master Mix Probe-based real-time PCR detection reagent
PRO
AffiQ™ Universal Probe SuperMix Universal qPCR mix for quantitative detection workflows
View qPCR Master Mix Probe

Rapid PCR Kits

Rapid PCR kits are optimized to shorten amplification time while maintaining reliable performance.

Rapid PCR Fast PCR kit Quick amplification
FAST
AffiFast™ Rapid PCR Master Mix Fast cycling PCR reagent for reduced amplification time
30
AffiFast™ Quick PCR Kit Rapid DNA amplification for time-sensitive workflows
View Rapid PCR

RT-qPCR SuperMix

RT-qPCR SuperMix products simplify RNA quantification workflows by combining optimized reagents.

RT-qPCR SuperMix RNA detection
RTQ
AffiRT-qPCR™ SuperMix Optimized reagent mix for RNA target quantification
2X
AffiRT-qPCR™ 2X Master SuperMix Ready-to-use format for reproducible RT-qPCR setup
View RT-qPCR SuperMix

How to Choose the Right PCR Kit

The best PCR kit depends on your sample type, target molecule, amplification speed, accuracy requirement and detection method.

Research Need Recommended Category Reason
Routine DNA amplification Conventional PCR Reliable endpoint PCR for standard molecular biology workflows
RNA target detection RT-PCR Kit or Reverse Transcriptase Converts RNA into cDNA before amplification
Fast sample screening Direct PCR or Rapid PCR Reduces preparation time and speeds up amplification
Cloning or sequencing preparation High-Fidelity PCR Improves sequence accuracy using proofreading polymerase activity
Quantitative DNA detection qPCR Master Mix Probe Supports real-time detection using fluorescent probes
RNA quantification One-Step RT-qPCR Mix or RT-qPCR SuperMix Combines reverse transcription with real-time quantitative PCR
miRNA expression analysis miRNA Reverse Transcription and miRNA qPCR Optimized for small RNA and microRNA detection workflows
Host cell residual DNA testing Host Cell Residual DNA Quantitative Detection Designed for sensitive residual DNA quantification

PCR, RT-PCR and qPCR FAQ

Frequently searched questions about PCR kits, RT PCR, qPCR, PCR reagents and molecular biology workflows.

What is PCR?
PCR stands for polymerase chain reaction. It is a molecular biology technique used to amplify specific DNA sequences through repeated temperature cycles. PCR is commonly used in DNA amplification, genotyping, cloning, sequencing preparation and research testing workflows.
What is a PCR kit?
A PCR kit contains optimized reagents for DNA amplification, usually including DNA polymerase, reaction buffer, dNTPs, magnesium and other components required for reliable polymerase chain reaction workflows.
What is RT-PCR?
RT-PCR means reverse transcription polymerase chain reaction. It is used when the starting material is RNA. RNA is first converted into complementary DNA, also called cDNA, using reverse transcriptase, and then amplified by PCR.
What is qPCR?
qPCR, or quantitative PCR, measures DNA amplification in real time using fluorescence. qPCR master mixes are used for quantitative nucleic acid detection, gene expression analysis, residual DNA detection and probe-based real-time PCR assays.
What is the difference between PCR, RT-PCR, qPCR and RT-qPCR?
PCR amplifies DNA. RT-PCR converts RNA into cDNA before amplification. qPCR measures DNA or cDNA amplification in real time. RT-qPCR combines reverse transcription with quantitative PCR for RNA target quantification.
Which PCR kit should I choose?
Choose conventional PCR for routine DNA amplification, RT-PCR kits for RNA targets, high-fidelity PCR for cloning or sequencing preparation, rapid PCR for faster workflows, and qPCR master mix or RT-qPCR SuperMix for quantitative detection.
What is direct PCR used for?
Direct PCR is used to amplify DNA from minimally processed samples. It helps reduce sample preparation time and is useful for fast screening, colony PCR, genotyping and high-throughput research workflows.
What is high-fidelity PCR?
High-fidelity PCR uses proofreading DNA polymerases to reduce amplification errors. It is recommended for cloning, sequencing preparation, mutagenesis, gene construct verification and applications where sequence accuracy is important.
What is miRNA qPCR?
miRNA qPCR is used to quantify microRNA expression. Because miRNAs are short RNA molecules, they require optimized reverse transcription and qPCR reagents for sensitive small RNA detection.
What are host cell residual DNA detection kits?
Host cell residual DNA quantitative detection kits are used to measure residual host cell DNA in research, bioprocess development and analytical workflows. These assays often use qPCR for sensitive DNA quantification.

Need Help Choosing the Right PCR Kit?

Affigen provides PCR kits, RT-PCR reagents, qPCR master mixes, direct PCR kits, high-fidelity PCR products, rapid PCR kits, miRNA qPCR reagents, RT-qPCR SuperMix and host cell residual DNA detection solutions for research laboratories.

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von Mariem BEN CHOIYA

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