SKU: AFG-VZ-MR101-01

AffiPCR® miRNA 1st Strand cDNA Synthesis Kit (by stem-loop)

Vendor AffiGEN
Regular price €112,00 EUR
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AffiPCR® miRNA 1st Strand cDNA Synthesis Kit (by stem-loop)

AffiPCR® miRNA 1st Strand cDNA Synthesis Kit (by stem-loop) is a specific kit for stem-loop miRNA cDNA one-strand synthesis. It includes a genomic DNA removal step, which can quickly remove the contamination of genomic DNA at 42°C for 2 min. This ensures that subsequent results are more reliable. The HiScript II Reverse Transcriptase on which the Kit is based has extremely high thermal stability, and with an optimized buffer system, it is beneficial to the generation of miRNA-specific reverse transcriptase products. For subsequent quantification of cDNA products, AffiGEN's miRNA Universal SYBR qPCR Master Mix (AffiGEN #AFG-VZ-MQ101) is recommended to obtain optimal experimental results.


Component 50 Reactions (20 µL/Reaction) 100 Reactions (20 µL/Reaction)
RNase-free ddH2O 1 mL 1 mL
5X gDNA Wiper Mix 100 μL 200 μL
10X RT Mixa 100 μL 200 μL
HiScript II Enzyme Mixb 100 μL 200 μL



This kit is suitable for one-strand synthesis of miRNA and cDNA by stem-loop method.


  • Prevent RNase contamination Keep the experimental area clean; wear clean gloves and masks during operation; consumables such as centrifuge tubes and pipette tips used in the experiment must be RNase-free.
  • Use of 5X gDNA Wiper Mix The 5X gDNA Wiper Mix contains a high concentration of glycerol. Before use, please briefly centrifuge to collect it at the bottom of the reaction tube, and mix by gently pipetting with a pipette. The pipette tip should not be inserted too far into the liquid surface, otherwise the amount of enzyme will be lost due to sticking of the pipette tip wall.
  • The preparation of the reaction solution should be completed on ice.

Storage Conditions:

Store at temperatures between -30°C and -15°C, and ensure transportation at ≤0°C. After thawing, they can be stored at 4°C for up to three months, maintaining their quality and efficacy.


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