SKU: AFG-VZ-P222-01

AffiPCR® 2X Rapid Taq Master Mix

Vendor AffiGEN
Regular price €107,00 EUR
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AffiPCR® 2X Rapid Taq Master Mix

AffiPCR® 2X Rapid Taq Master Mix has been developed to optimize PCR for faster and more efficient results. It contains the required components for robust amplification with high yield and sensitivity, allowing you to get reliable, reproducible results in less time.

AffiPCR® 2X Rapid Taq Master Mix contains Taq DNA Polymerase, elongation promoting factor, dNTP and an optimized buffer system. The amplification speed of this product can reach 15 sec/kb, which is suitable for rapid PCR. The maximum amplification speed within 1 kb can reach 1 sec/kb, greatly saving reaction time. The pre-prepared 2X Master Mix only needs to add primers and templates to perform amplification, which reduces pipetting operations and improves detection throughput and results reproducibility. The kit has excellent amplification performance and high storage stability. It is suitable for PCR amplification within 5 kb using genome as template and PCR amplification within 10 kb using plasmid and λDNA as template. The protective agent added to the system allows 2X Master Mix to maintain stable activity after repeated freezing and thawing. The kit provides a version containing electrophoresis buffer and green loading dye, which can be directly electrophoresed after the reaction that is convenient to use. The PCR product has a poly(A) tail at the 3' end, which can be directly cloned into T vectors and is suitable for ClonExpress and TOPO cloning kits.

Storage Conditions:

Store at temperatures between : -30°C and -15°C, and ensure transportation at ≤0°C. After thawing, they can be stored at 4°C for up to three months, maintaining their quality and efficacy.


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