SKU: AFG-VZ-R223-01

AffiPCR® RT SuperMix for qPCR (+gDNA wiper) V2

Vendor AffiGEN
Regular price €319,00 EUR
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AffiPCR® RT SuperMix for qPCR (+gDNA wiper) V2

AffiPCR® HiScript II Reverse Transcriptase is a novel reverse transcriptase obtained by in vitro molecular evolution technology, based on M-MLV (RNase H-) Reverse Transcriptase, HiScript II Reverse Transcriptase greatly improves the thermal stability and cDNA synthesis efficiency. 
The HiScript II Q RT SuperMix for qPCR (+gDNA wiper) is specially designed for two-step qRT-PCR. The residual genomic DNA in RNA template can be removed rapidly with the 4X gDNA Wiper Mix. The 5X HiScript qRT SuperMix II contains all necessary components required for reverse transcription. With the addition of template RNA and ddH2O, the reaction can proceed quickly, meanwhile, gDNA wiper is terminated to ensure the integrity of the cDNA.
This product has been specially optimized for qPCR. With the optimized proportion of Random primers/Oligo (dT) 23VN primer mix, cDNA synthesis can start from each region of RNA transcript and have the same reverse transcription efficiency, which ensures the authenticity and reproducibility of qPCR results. The RT product is compatible with dye-based and probe-based qPCR, and can be used with the corresponding reagent according to the experimental purpose to perform high-performance gene expression analysis.


Component AFG-VZ-R223-01
100 rxns (20 μl/rxn)
RNase free ddH2O 2 × 1 ml
4X gDNA wiper Mix 400 μl
5X HiScript II qRT SuperMix IIa 400 µl
5X No RT Control Mixb 40 µl


a. It contains Buffer, dNTPs, HiScript II Reverse Transcriptase, RNase inhibitor, and Random primers/Oligo (dT)23 VN primer Mix.
Different from the 5X HiScript II qRT SuperMix in HiScript II Q RT SuperMix for qPCR (AffiGEN #AFG-VZ-R222), they cannot be mixed.
b. It does not contain HiScript II reverse transcriptase. Other components are the same as 5X HiScript II qRT SuperMix for the preparation of No RT Control reaction.



Store at -30 ~ -15°C and transport at ≤0°C.


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