AffiAB® Goat Anti-RFP, DyLight®550 Polyclonal IgG Antibody provides a reliable and consistent solution for analyzing and visualizing target proteins using fluorescence-based detection systems. This polyclonal antibody has been tested and verified to be highly specific and sensitive for the detection of RFP proteins. The DyLight®550 dye offers excellent photostability and signal-to-noise ratio for robust imaging.
The AffiAB® Goat Anti-RFP, DyLight®550 Polyclonal IgG Antibody is an antibody specifically designed to target and bind to RFP (Red Fluorescent Protein) when conjugated with DyLight®550, an orange-red fluorescent dye. This antibody is produced by immunizing goats with RFP protein or peptides, and the resulting polyclonal antibody is purified and conjugated with the DyLight®550 dye.
RFP is a commonly used fluorescent protein derived from Discosoma sp. coral, which emits red fluorescence when excited by appropriate wavelengths of light. By conjugating the anti-RFP antibody with DyLight®550, the resulting antibody can be excited by orange or red light and emit an orange-red fluorescence signal when bound to RFP-tagged proteins.
The AffiAB® Goat Anti-RFP, DyLight®550 Polyclonal IgG Antibody is frequently used in fluorescence-based techniques such as immunofluorescence, immunocytochemistry, and flow cytometry. It enables researchers to specifically detect and visualize RFP-tagged proteins in biological samples using orange-red excitation and detection channels.
When working with this antibody, it is important to follow the recommended protocols provided by the manufacturer, including sample preparation, antibody dilution, and incubation times. Additionally, proper controls and optimization steps should be performed to ensure specific binding and minimize background signals.
In summary, the AffiAB® Goat Anti-RFP, DyLight®550 Polyclonal IgG Antibody is a valuable tool for researchers studying RFP-tagged proteins who require an orange-red fluorescent detection system. It allows for the specific visualization and analysis of RFP-tagged proteins in various experimental setups, contributing to the understanding of cellular processes and protein dynamics.