AffiAB® Goat anti-Cre Polyclonal IgG Antibody
AffiAB® Goat anti-Cre Polyclonal IgG Antibody is a highly specific antibody designed for use in a wide range of research applications. The antibody is produced in goat and specifically targets the Cre gene product. It is optimized for use in western blot and immunohistochemistry protocols.
Host: Goat
Species: N/A
Description:
AffiAB® Goat anti-Cre Polyclonal IgG Antibody is an antibody produced by goats that specifically targets the Cre recombinase protein. Cre recombinase is an enzyme that is commonly used in genetic engineering to excise or insert DNA segments in a specific region of a genome. The antibody can be used to detect the presence and localization of Cre recombinase in cells or tissues through immunostaining or western blotting techniques. The AffiAB® brand is known for producing highly specific and reliable antibodies for research use.
Gene Identifier/Accession Number: N/A
Form: N/A
Concentration: 2,5 mg/mL
Type: Primary
Clonality: Polyclonal
Isotype: IgG
Conjugation: Unconjugated
Application: Western Blot
Storage: For continuous use, store at 2-8 °C for one-two days. For extended storage, store in -20 °C freezer. Working dilution samples should be discarded if not used within 12 hours.
Special instructions: The antibody solution should be gently mixed before use.
Goat anti-Cre Polyclonal IgG Antibody
The Goat Anti-Cre Polyclonal IgG Antibody is a powerful tool in molecular biology and genetic research. Its ability to specifically bind to the Cre recombinase enzyme, a pivotal element in the Cre-loxP recombination system, makes it invaluable in various research settings. This article delves into the properties, applications, and significance of this specialized antibody, highlighting its role in advancing scientific understanding.
Understanding Goat Anti-Cre Polyclonal IgG Antibody
Production and Specificity:
Goat Anti-Cre Polyclonal IgG Antibody is produced by immunizing goats with a Cre recombinase protein. This process results in the animal's immune system producing IgG antibodies that are specific to the Cre protein. Polyclonal antibodies, like this one, are known for recognizing multiple epitopes on an antigen, enhancing their binding efficiency and sensitivity.
Advantages of Goat-Derived Antibodies:
Goat-derived antibodies are preferred in certain research scenarios due to their high yield and the robust immune response goats can provide. This results in the production of antibodies that are often more sensitive and have a broader range of reactivity compared to those derived from other species.
Applications in Research
1. Gene Expression Studies:
The Goat Anti-Cre Polyclonal IgG Antibody is instrumental in studying gene expression in organisms where the Cre-loxP system has been employed. It allows researchers to track and analyze the expression of Cre recombinase, providing insights into gene regulation and function.
2. Transgenic Animal Models:
In transgenic animals, where the Cre-loxP system is used for gene editing, this antibody helps in monitoring and verifying the successful expression of Cre. This is crucial in studies related to genetics, developmental biology, and disease modeling.
3. Cell Line Development:
In cell culture, the antibody can be used to confirm the presence and activity of Cre recombinase in cells engineered to express this enzyme. This is essential for validating the efficiency of gene editing techniques.
4. Immunohistochemistry (IHC):
In IHC, the antibody aids in detecting Cre recombinase in tissue samples, providing valuable information about the spatial and temporal patterns of gene expression in different tissues.
5. Western Blotting:
It is also a key tool in Western blotting assays to detect and quantify the Cre protein in various sample types, ensuring precise and reliable results.
Conclusion
The Goat Anti-Cre Polyclonal IgG Antibody is a remarkable tool in the field of molecular biology and genetic research. Its specificity, sensitivity, and versatility make it essential for researchers exploring the complexities of gene expression and regulation. As scientific exploration advances, tools like this antibody play a crucial role in enhancing our understanding of genetic mechanisms and their applications in health and disease.