AffiVET® Bovine Pregnancy Rapid Test Kit

SKU: AFG-VR-84

AffiVET® Bovine Pregnancy Rapid Test Kit

AffiGEN
$198.00 USD
$198.00 USD
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AffiVET® Bovine Pregnancy Rapid Test Kit

Cat Number: AFG-VR-84
Size: 25 Tests
Species: Bovine
Sample: Whole blood, Serum and Plasma

Product Datasheet

Overview:

Detecting pregnancy early is crucial in modern animal husbandry, especially with the prevalence of artificial insemination. It allows for timely adjustments in feeding plans, reducing costs associated with feed and labor. Traditional methods like observation or ultrasound are costly and impractical for smaller farms. Enter the bovine pregnancy rapid test kit: a 15-minute lateral flow immunoassay leveraging monoclonal antibodies targeting bovine pregnancy-associated glycoproteins (PAGs). This kit efficiently identifies PAG concentration, providing a swift and economical means of determining pregnancy status in bovines.

Material Required:

(25) Rapid Test Card in foil pouch.

(50) Pipette

(25) Sample Buffer Tubes 

(25) Needle and EDTA blood tube

(01) Kit instruction

Test Application: 

The AffiVET® Bovine Pregnancy Rapid Test Kit is designed specifically for detecting bovine pregnancy in serum or plasma samples starting from 28 days post-mating or artificial insemination (AI). For whole blood samples, it's advisable to conduct the test at 35 days post AI. However, testing shouldn't be carried out until 70 days after calving due to the extended half-life of the target.

Storage & Stability:

  • Store at 2-30°C in cool dark place, do not freeze.
  • The kit is valid for 18 months.

Sample collection & Preparation:

Whole blood:

  • Draw blood from cattle at least 35 days post AI using EDTA blood tubes.
  • Ensure thorough mixing by gently inverting the tube 10 times immediately after collection.
  • Store the whole blood at 2-8°C for up to one day; however, freezing is not recommended.
Serum:
  • Collect blood from cattle at least 28 days post AI in tubes without anticoagulant.
  • Process the blood to obtain serum by centrifugation at 5000rpm for 5 minutes.
  • Use the upper clear liquid as serum and store it at 2-8°C for no more than one day.
Plasma:
  • Collect blood in EDTA-containing tubes (provided in the kit) and mix immediately by inversion.
  • Process the tubes to obtain plasma by centrifugation at 5000rpm for 5 minutes.
  • Utilize the upper clear liquid as plasma and store it at 2-8°C for no more than one day.

Test Procedure:

  1. Before beginning the experiment, carefully review the instructions. Ensure all samples are freshly collected. Allow both the test kit and the test samples to reach room temperature (20-30°C).
  2. Retrieve the required test card from the kit package and make appropriate markings. Utilize the test strips within one hour. Any unused strips should be promptly returned to cold storage.
  3. For serum/plasma samples: Add 3 drops (90-120μl) of serum/plasma sample into the sample well on the test card.
  4. For whole blood samples: Add 1 drop (40ul) of whole blood into the sample well on the test card, then add 2 drops (60ul) of sample buffer using the buffer vial provided in the kit.
  5. Wait for 15 minutes, then proceed to determine the result according to Part 5 of the instructions. Any results observed after 30 minutes are considered invalid.

Result Interpretation:

Negative result

Presence of C-Line
Absence of T-Line

This means that there is no bovine pregnancy-associated glycoproteins (PAGs) in the sample or the level is lower than the test detection limit.

Positive result:

Presence of C-Line
Presence of T-Line

This confirms the presence of the bovine pregnancy-associated glycoproteins (PAGs) in the sample.

Invalid Result:

Absence of C-Line
Presence of T-Line

Or

Absence of C-Line
Absence of T-Line

This means that the procedure was not applied correctly or that the test is invalid.

NOTES :
  • Positive result needs to be confirmed by testing of sample from the cow on the next day.
  • In field test of more than 500 cows, this kit demonstrated 98.3% specificity and 97.5% sensitivity. The total accuracy is over 97%. However, since this is not a confirmatory test, positive results shall be confirmed by other methods.

Cautions:

1. On day 28 post AI, positive results will display a faint or weak test line. If a weak or very weak test line is visible, the result is positive.

2. The visibility of the test line improves around day 35-40 post AI when the Pregnancy-Associated Glycoprotein (PAG) levels in blood increase. Therefore, it's recommended to collect blood samples on day 35 or later post AI.

3. Test lines are more prominent on test cards using serum/plasma samples due to direct sample application without buffer dilution.

4. Test lines are less visible on test cards using whole blood samples because of dilution with sample buffer. Testing on day 35 or later ensures a clearer test line.

5. Test results with incomplete test lines or control lines are still valid.

6. Collaboration with a veterinarian to establish protocols using this test kit on your farm is recommended. For example, conducting plasma or serum testing on day 28 post AI can help identify all negative cows (open cows). For these open cows, repeat AI procedures. Repeat testing on day 28 again.

7. This test kit complements ultrasound scanners; it enhances testing efficiency and improves AI timing but doesn't replace them.

8 .To ensure accurate results, conduct the assay following the provided instructions. Utilize the test card within one hour of opening the foil pouch. Wear gloves during the testing process.

9. The components of this test kit are single-use only and should not be reused under any circumstances.

10. Collaborate with a veterinarian to establish appropriate reproductive programs, optimizing the utilization of the test kit for enhanced effectiveness.

Limitations: 

The kit is designed for the fast detection of pregnancy in cows, relying on the levels of Pregnancy-Associated Glycoproteins (PAG). However, it's important to note some limitations and considerations:

1. Post Parturition Limitation: The kit cannot be effectively used for cows that are 60 days post-parturition.

2. Relation to Embryo Viability: PAG levels are indicative of embryo viability. However, there may be instances where the embryo stops developing early in pregnancy, leading to a delay in PAG concentration change. This could result in a positive test followed by a negative test in subsequent days.

3. Sensitivity in Field Testing: Serum or plasma samples collected between day 28 and day 34 post Artificial Insemination (AI) or mating have demonstrated higher sensitivity for pregnancy testing. Specifically, at 32-34 days post AI, the sensitivity exceeds 99.5%.

4. Accuracy in Detecting Open Cows: The kit is particularly accurate in detecting open cows at 28 days post AI, with an accuracy of over 99.1%. Negative results at 28 days with this kit reliably indicate non-pregnancy, which remains consistent with ultrasound scanners at 32-35 days post AI.

5. Recommendations for Practical Use: Based on field testing data, it's recommended to integrate this kit into a customized reproduction program with veterinary guidance. It should complement, not replace, ultrasound scanners. For example, utilize the kit on day 28 post AI to identify non-pregnant cows for subsequent AI, and on days 32-35 to monitor embryo or fetus viability.

6. Applicability: The kit is suitable for use with female cattle, including dairy and beef cattle, but not for bulls. All components within the kit are non-contagious and can be safely handled.

In summary, while the kit offers a valuable tool for pregnancy detection in cows, its effectiveness is optimized when integrated into a comprehensive reproduction program under veterinary supervision.