SKU: AFG-VZ-P515-01

AffiPCR® 2X Max Master Mix

Vendor AffiGEN
Regular price $101.00 USD
Sale price $101.00 USD Regular price
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AffiPCR® 2X Max Master Mix

Get the most out of your PCR experiments with AffiPCR® 2X Max Master Mix This powerful master mix includes two tubes of AffiPCR® Max reagents, specifically designed to provide maximum sensitivity and specificity in PCR. Our specialized formulation increases primer annealing efficiency, reducing the amount of unsuccessful experiments. Get ready for more successful PCR results with AffiPCR® Max Master Mix.

AffiPCR® 2X Max Master Mixis an upgraded versionMax Master Mix. Compared with the previous generation,it is the unique elongation factor, specificity-enhancing factor and plateau phase anti-inhibitor factor, which greatly improves the long-fragment amplification ability, amplification specificity and amplification yield. AffiPCR® Max can efficiently amplify up to 40 kb simple templates (e.g. λDNA, plasmids), 20 kb complex templates (e.g. genomic DNA) and 10 kb cDNA. The amplification error rate of AffiPCR® Max is 128-fold lower than that of conventional Taq DNA Polymerase. In addition, AffiPCR® Max has a good resistance to PCR inhibitors and can be used for direct PCR amplification of bacteria, fungi, plant tissues, animal tissues, and even whole blood samples. AffiPCR® Max contains two monoclonal antibodies inhibiting the 5'→3' polymerase activity and 3'→5' exonuclease activity at room temperature, which enable it to perform hot start PCR with great specificity. This product contains AffiPCR® Max Super-Fidelity DNA Polymerase, dNTP, and an optimized buffer system. It only needs to add primers and templates to perform amplification, thereby reducing pipetting operations and improving detection throughput and reproducibility of results. The system contains protective agents that keep 2X AffiPCR® Max Master Mix stable in activity after repeated freezing and thawing. Amplification products are blunt-ended, which are compatible with ClonExpress kits.

Component:

Components AFG-VZ-P515-01 AFG-VZ-P515-02 AFG-VZ-P515-03
2 X Max Master Mix 1 mL 5 × 1 mL  15 × 1 mL

 

Equivalence:

AFG-VZ-P505 AffiGEN AffiPCR® Max Super-Fidelity DNA Polymerase
AFG-VZ-515 AffiGEN AffiPCR® 2X Max Master Mix
AFG-VZ-525 AffiGEN AffiPCR® 2X Max Master Mix (Dye Plus)
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KOD-401 TOYOBO KOD-Plus-Neo
KFX-201 TOYOBO KOD FX Neo
M0493S NEB Q5® Hot Start High-Fidelity DNA Polymerase
M0494S NEB Q5® Hot Start High-Fidelity 2X Master Mix
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RR371A Takara Ex Premier Polymerase Dye plus
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12344024 Invitrogen AccuPrime™ Pfx DNA Polymerase