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AffiGEN® TransfectTM DNA In Vitro 2000 V1
Product Description
AffiGEN® TransfectTM DNA In Vitro 2000 V1 is a potent transfection reagent that ensures highly efficient and consistent transfection outcomes while maintaining minimal toxicity. It utilizes a unique chemical formulation involving the covalent cross-linking of cationic lipids with polymers, resulting in remarkable transfection efficiency and distinct advantages over other types of transfection reagents. AffiGEN® TransfectTM DNA In Vitro 2000 V1 has demonstrated its ability to successfully deliver genes to a diverse range of cell lines, both established and primary, including HEK293, 293T, 293E, CHO, COS1, HeLa, NIH 3T3, insect cell lines such as Sf9 and Sf21, and various other eukaryotic cell lines. One unit of AffiGEN® TransfectTM DNA In Vitro 2000 V1, with a volume of 1.0 ml, is suitable for more than 600 transfections in 24-well plates or around 333 transfections in 6-well plates.
Application
- Facilitates the delivery of DNA/RNA into mammalian and insect cells.
- Dramatically enhances virus infection efficiency (up to 200 times) for challenging-to-transfect cells, including replication-deficient Adenovirus.
Features
- Exceptionally high virus production yields.
- Equally effective for very long DNA molecules (up to 56 kb).
- Suitable for both single and multiple DNA transfections.
- Achieves exceptionally high levels of recombinant protein production.
- The preferred choice for challenging-to-transfect cells.
- A cost-effective transfection reagent.
Storage Condition
Store AffiGEN® TransfectTM DNA In Vitro 2000 V1 at 4°C. When stored correctly, the product maintains stability for 12 months or longer.
DNA In Vitro Transfection Reagents Specifications
Product | Nucleic Acid | Type | Delivery Method | Toxicity | Efficiency | Serum Tolerance |
AffiGEN® Transfect™ 1000 - Mammalian Cell | DNA | Calcium phosphate | Precipitation | High | High | High |
AffiGEN® Transfect™ DNA In Vitro 2000 V1 | DNA, siRNA | Polymer | Polymer complex | Low | High | Low |
AffiGEN® Transfect™ DNA In Vitro 2000 V2 | DNA, siRNA | Polymer | Polymer complex | Moderate | Higher | Medium |
AffiGEN® Transfect™ DNA In Vitro 2000 Plus | DNA | Polymer | Polymer complex | Low | Higher | Medium |
AffiGEN® Transfect™ DNA In Vitro 3000 | DNA | Liposomal | Liposome-mediated | Moderate | High | High |
AffiGEN® Transfect™ DNA & siRNA In Vitro 4000 Kit | DNA, siRNA | Liposomal | Liposome-mediated | Very low | High | High |
AffiGEN® Transfect™ DNA In Vitro 5000 | DNA, siRNA | Polymer | Polymer complex | Moderate | High | High |
DNA In Vitro Transfection Reagents Application Guide
Product | Applications | Cell Types | Cell lines | Suitable for | Additional Notes |
AffiGEN® Transfect™ 1000 - Mammalian Cell | Protein production, stable cell line generation, viral vector production | Adherent, some suspension | HEK293, HepG2, CHO | Large-scale transfection, cost-effective |
Lower calcium concentration than traditional methods
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AffiGEN® Transfect™ DNA In Vitro 2000 V1 | Gene expression studies, protein production, virus production | Most, including sensitive | Various | Sensitive cell lines, maximizing cell viability |
Multiple formulations available
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AffiGEN® Transfect™ DNA In Vitro 2000 V2 | Gene expression studies, protein production, virus production | Most, including sensitive | Various | Balancing efficiency and viability |
Released from DNA complex
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AffiGEN® Transfect™ DNA In Vitro 2000 Plus | Protein production, viral vector production | Most, including sensitive | Various | Long DNA, high efficiency |
Proprietary polymer
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AffiGEN® Transfect™ DNA In Vitro 3000 | Viral vector production, antibody production, protein production | Adherent, suspension | Various | Lentivirus production, high efficiency |
Enhanced conjugate
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AffiGEN® Transfect™ DNA & siRNA In Vitro 4000 Kit | Protein production, gene editing, virus production | Most, including sensitive | Various | Low toxicity, high efficiency |
Fluorinated cationic lipids
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AffiGEN® Transfect™ DNA In Vitro 5000 | Gene expression studies, protein production, virus production | Most, including sensitive | Various | Common cell lines, biodegradable |
Biodegradable polycationic polymer
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