AffiDETECT® TUNEL BrightRed Apoptosis Detection Kit
During apoptosis, intracellular specific endonucleases are activated, chromatin DNA is specifically cleaved between nucleosomes, and DNA is degraded into integer multiples of about 180 - 200 bp fragments. The 3'-hydroxyl (3'-OH) end generated by broken DNA can bind to tetramethylrhodamine-deoxyuridine triphosphate (TMR red-dUTP) under the action of terminal deoxynucleotidyl transferase (TdT). Broken DNA labeled with TMR red-dUTP can be directly observed by fluorescence microscopy or quantitatively analyzed by flow cytometry to reflect the level of apoptosis. The BrightRed Labeling Mix contains TMR red-dUTP and Bright factor. This unique small molecule compound can non-covalently bind to TMR red-dUTP, enhance its stability and amplify its signal, resulting in brighter labeled fluorescence and stronger anti-quenching ability.
Storage Conditions:
Store at -30 ~ -15℃ and transport at ≤0℃. BrightRed Labeling Mix: Store at -30 ~ -15℃ and protect from light.
Applications:
It is applicable for apoptosis detection of paraffin sections, frozen sections, adherent and suspension cultured cells.
Materials:
Reagent: 4% paraformaldehyde, DAPI, Triton X-100, PBS, absolute ethanol. Other materials: coverslips/slides, wet box.
Note:
For research use only. Not for use in diagnostic procedures.