AffiCLONE® Ultra One Step Cloning Kit
The AffiCLONE® Ultra technology is simple, fast, and highly efficient DNA seamless cloning technology. It enables rapid directional cloning of inserts into any site in any vector. Use any method to linearize the vector, and introduce the end sequence of the linearized vector at the 5' end of the insert forward/reverse amplification primer, so that the 5' and 3' ends of the PCR product have the same ends sequence (15 - 20 bp) as the linearized vector, respectively. The PCR product with the same sequence as the end of the vector and the linearized vector are mixed in a certain proportion. Under the catalysis of recombinase, the transformation can be performed at 50℃ for 5 - 15 min to complete the directional cloning. AffiCLONE® Ultra One Step Cloning Kit is a new generation of recombinant cloning kits, compatible with 1 - 5 fragments homologous recombination. The unique ligase-independent system of this kit significantly reduces the self-ligation background of the vector. Restriction endonuclease sites do not need to be considered. Highly optimized 2X AffiCLONE® Mix (It contains enhanced recombinase Exnase) can significantly improve the recombination efficiency and the tolerance to impurities. pCE-Zero vector is compatible with most PCR products, enabling the specific PCR products to be used directly for recombination without any treatments, which significantly simplify the procedure.
Components:
| Components | 25 Reactions | 50 Reactions |
| 2X AffiCLONE® Mix | 125 µL | 2 X 125 µL |
| pCE-Zero vector, linearized (50 ng/μl)* | 25 µL | 50 µL |
| 500bp control insert (20 ng/μl） | 5 µL | 5 µL |
Application:
- Fast Cloning
- High-throughput
- Cloning Seamless
- Cloning DNA Site-directed Mutagenesis