AffiASSAY® MTS Cell Proliferation Colorimetric Assay Kit

AffiASSAY® MTS Cell Proliferation Colorimetric Assay Kit

AffiASSAY® MTS Cell Proliferation Colorimetric Assay Kit is designed to provide accurate and reliable measurements of cell proliferation in various biological samples. This assay kit offers a robust tool for researchers in the fields of cell biology, pharmacology, and drug discovery.

Key Features:

• High Sensitivity: Our assay kit is optimized to detect subtle changes in cell proliferation, enabling precise quantification across a wide range of conditions.
• Colorimetric Detection: Leveraging the colorimetric assay principle, our kit ensures reproducible results with minimal background interference, enhancing assay reliability.
• Versatile Application: The AffiASSAY® MTS Cell Proliferation Colorimetric Assay Kit is compatible with diverse cell types and culture conditions, facilitating broad experimental applicability.
• Straightforward Protocol: With a user-friendly protocol, you can obtain proliferation data efficiently, saving time and resources in your research endeavors.
• Reliable Performance: Whether assessing compound cytotoxicity, screening for proliferation modulators, or studying cellular responses, our kit delivers consistent and dependable results.

Assay Principle:

This assay kit utilizes the reduction of the yellow tetrazolium salt MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) to formazan by metabolically active cells. The formazan product's absorbance is proportional to the number of viable proliferating cells in the sample, allowing accurate quantification of cell proliferation.

Applications:

• Cell Viability Assessment: Evaluate the viability and proliferation potential of cultured cells under different experimental conditions.
• Drug Screening: Screen compounds for their effects on cell proliferation, aiding in the identification of potential therapeutic agents.
• Proliferation Assays: Study cellular proliferation dynamics in response to stimuli, treatments, or genetic manipulations with precision.
• Cellular Toxicity Testing: Assess the cytotoxicity of chemicals, drugs, or environmental factors on cell growth and proliferation.

Kit Contents:

• MTS Reagent
• Cell Culture Medium
• Stop Solution
• 96-Well Assay Plate
• Detailed Protocol

Why Choose AffiASSAY®?

Our dedication to scientific excellence and quality assurance makes AffiASSAY® the preferred choice for researchers worldwide. We are committed to providing innovative solutions that empower your research and accelerate scientific discoveries.

AffiASSAY® MTS Cell Proliferation Colorimetric Assay Kit Protocol:

[Please note that this protocol serves as a general guideline. Always refer to the specific instructions provided in the kit manual for precise details.]

Materials Needed:

• AffiASSAY® MTS Cell Proliferation Colorimetric Assay Kit
• Cultured cells or tissue samples
• Cell culture plates or vessels
• 37°C CO₂ incubator
• Microplate reader capable of measuring absorbance at ~490 nm
• Pipettes and tips

Protocol:

Cell Preparation:

• Culture cells according to standard protocols until reaching the desired confluence or experimental stage.
• Harvest cells using trypsin or a suitable cell dissociation reagent and resuspend them in fresh culture medium.
• Adjust cell density as needed for the assay.

Assay Plate Setup:

• Seed cells in a 96-well plate at appropriate densities, ensuring uniform distribution across wells.
• Include wells for blank controls (medium only) and positive controls (untreated cells) as references.

MTS Assay:

• Prepare the MTS reagent according to the kit instructions.
• Add an appropriate volume of MTS reagent to each well containing cells.
• Incubate the plate at 37°C in a CO₂ incubator for a specified duration, typically 1-4 hours.
• After incubation, stop the reaction by adding the provided stop solution.

Optical Density Measurement:

• Measure the absorbance of the formazan product at around 490 nm using a microplate reader.

Data Analysis:

• Calculate cell proliferation or viability based on the absorbance readings, comparing experimental wells to controls.
• Plot data and analyze results according to your research objectives.

Results Interpretation:

• Interpret assay results in the context of your experimental conditions and hypotheses.
• Consider factors such as treatment effects, time course, and replicates in result interpretation.

Data Presentation:

• Prepare figures or tables summarizing your findings for presentation or publication.
• Include statistical analyses if applicable to enhance result credibility.

Cleanup:

• Dispose of all waste materials properly, following institutional guidelines for biohazardous waste.
• Ensure proper storage and labeling of remaining kit components for future use.