AffiNGS® Discover-sc Single Cell WGA Kit
The AffiNGS® Discover-sc Single Cell WGA Kit is based on multiple displacement amplification (MDA) and is designed for unbiased whole genome amplification from single cell and other micro sample.
The size of amplification products are between 2 kb to 100 kb, with a coverage greater than 95% and an average length higher than 15 kb. The products can be widely applied to whole genome sequencing, whole exome sequencing, marge fragment copy number variation analysis, microsatellite analysis, qPCR analysis, or gene chip analysis.
The Phi29 DNA polymerase used in this kit is cloned from phage and has a strong strand displacement activity. Melting and replication of the DNA chain with complex structure can be achieved. Meanwhile, Phil29 has a strong chain affinity and can achieve up to 100 kb of continuous polymerization, and the amplification product can be applied to applied to almost all of the downstream genome analysis. Phi29 DNA polymerase also possesses a potent 3'→5' exonuclease (proofreading) activity, its fidelity is 1,000-fold that of Taq enzyme, thus ensuring the high fidelity of DNA replication.
All the reagents provided in the kit have undergone rigorous quality control and functional testing to ensure the optimal stability and repeatability.
Components :
| Components |
AFG-VZ-N603-01 (24 rxns) |
AFG-VZ-N603-02 (96 rxns) |
| Discover-sc WGA Enzyme Mix | 48 µl | 192 µl |
| Discover-sc WGA Master Buffer | 750 µl | 3 x 1 ml |
| Buffer D | 1 ml | 2 x 1 ml |
| Stop Solution | 1 ml | 2 x 1 ml |
| DTT, 1 M | 1 ml | 1 ml |
| Cell Storage Buffer | 1 ml | 2 x 1 ml |
| Nuclease-free ddH2O | 1 ml | 2 x 1 ml |
The color marked in the product components table represents the color of each tube lid.
Storage :
Store at -30 ~ -15° and transport at ≤0°C. For long-term storage, please store at-85 ~ -65°C.