Taking Control of Mycoplasma: A Complete 3-Step Lab Solution
How the AffiGEN Toolkit Protects Your Cells, Data, and Scientific Integrity
If you’ve ever noticed slow-growing cells, inconsistent experimental results, or unexplained variability, you might be dealing with a hidden threat: mycoplasma contamination.
Unlike bacterial or fungal contamination, mycoplasma doesn’t cloud your culture. It hides in plain sight — altering gene expression, metabolism, and reproducibility — all without obvious visual signs.
At AffiGEN, we’ve developed a complete and reliable 3-product system that enables labs to detect, confirm, and eliminate mycoplasma contamination efficiently, with minimal disruption to your workflow.
🔍 Step 1: Quick Screening with AffiZYME® MycoVisual Kit
Your first layer of protection is routine screening — and for that, there's no simpler tool than the AffiZYME® MycoVisual Kit.
This easy-to-use test is based on LAMP (Loop-mediated Isothermal Amplification) technology. It detects mycoplasma DNA in your cell culture supernatant through a clear color change — from purple-red to yellow — in just 60 minutes.
- No special equipment required
- Colorimetric visual readout
- Ideal for weekly screening
- Safe, sealed-tube format to avoid contamination
This kit is perfect for routine checks — offering peace of mind in minutes.
AffiZYME® MycoVisual Kit
Simple, color-based results in 60 minutes. Ideal for routine screening without qPCR equipment. 25 or 50 reactions.
Learn More🧪 Step 2: Confirm Results with AffiPCR® MycoDetect Kit
When a visual screen raises concern, you'll want quantitative confirmation — and that's where the AffiPCR® MycoDetect Kit steps in.
Powered by real-time qPCR, this high-sensitivity assay detects as few as 10 copies of mycoplasma DNA, delivering results in under 2 hours with high specificity.
- Ct-based detection (Ct < 35 = positive)
- Compatible with most qPCR platforms
- No DNA extraction required
- Built-in positive and negative controls
This kit ensures that your diagnosis is both accurate and actionable.
AffiPCR® MycoDetect Kit
qPCR kit for rapid and precise mycoplasma detection. Detects as few as 10 DNA copies. 100 reactions.
Learn More🧼 Step 3: Restore Clean Cultures with AffiCLEAN® MycoX Removal Mix
Once contamination is confirmed, the priority becomes restoring your cultures — safely and effectively. AffiCLEAN® MycoX Removal Mix is a concentrated reagent that removes over 70 strains of mycoplasma without damaging your cells.
- Non-toxic to common mammalian lines
- Effective within 2–3 weeks (4–6 passages)
- Simple 1:1000 dilution into culture media
- No need to discard valuable lines
With this reagent, labs can confidently recover important cell stocks and move forward without disruption.
AffiCLEAN® MycoX Removal Mix
Non-toxic reagent for effective mycoplasma removal from cell cultures. Works in 2–3 weeks. 1000× concentrate.
Learn More🧠 A Smarter Mycoplasma Control Workflow
Thousands of labs now follow this three-step strategy:
- Screen regularly with AffiZYME® MycoVisual Kit
- Confirm positive cases using AffiPCR® MycoDetect Kit
- Clean contaminated lines with AffiCLEAN® MycoX™ Removal Mix
- Re-test to verify complete removal
This integrated workflow helps ensure clean cultures, reliable data, and research continuity.
🔗 Build Confidence in Your Cultures
Mycoplasma contamination can compromise weeks or months of work. But with the right tools, it becomes a manageable part of routine quality control.
With the AffiGEN Mycoplasma Control Suite, you gain a proactive, lab-tested approach to protecting your research from this common — but often overlooked — issue.
✅ Simple. Reliable. Complete.
Explore the full suite:
- AffiZYME® MycoVisual Kit
- AffiPCR® MycoDetect Kit
- AffiCLEAN® MycoX™ Removal Mix
Let your lab stay focused on innovation, not contamination.