AffiCLEAN® MycoX™ Removal Mix
AffiCLEAN® MycoX™ Removal Mix is a powerful, broad-spectrum antimicrobial reagent designed to eliminate mycoplasma contamination from mammalian cell cultures. Its 1000× concentrated format allows economical and long-term use while maintaining high efficacy and low cytotoxicity. Ideal for both adherent and suspension cell lines, it enables complete decontamination typically within 2–3 weeks without affecting cell viability.
Key Features
- Broad-Spectrum Activity – Targets over 70 mycoplasma species
- Cell-Safe Formula – Non-cytotoxic to commonly used mammalian cells
- Fast Decontamination – Complete clearance within 4–6 passages
- Ready-to-Use Format – Simply dilute into culture medium (1:1000)
- Cost-Efficient – 1 mL treats up to 1 liter of culture
Applications
- Decontamination of mycoplasma-infected cell lines
- Rescue of valuable cell stocks without starting over
- Routine cleanup in academic, biotech, and GMP research labs
- Preparation of mycoplasma-free master cell banks
Kit Components
(Detailled kit components provided in the datasheet)
- (1 Vial) AffiCLEAN® MycoX™ Removal Mix
Recommended Protocol
(Detailled protocol provided in the datasheet)
Dilution: Add 1 μL of MycoX™ to 1 mL of culture medium (final 1×).
Treatment Schedule:
- Apply continuously for 4–6 cell passages (approx. 2–3 weeks)
- Replace media every 2–3 days with fresh MycoX™
Post-Treatment: Validate decontamination using AffiPCR® or AffiZYME® mycoplasma detection kits.
Storage & Stability
- Storage: 2–8°C (refrigerated)
- Shelf life: 12 months unopened
- Do not freeze
Quality Control
- Each batch is tested for sterility and mycoplasma elimination efficiency
- Validated against a panel of common Mycoplasma strains