AffiVET® Veterinary FMD NSP Antibody ELISA Kit

AffiVET® Veterinary FMD NSP Antibody ELISA Kit

Species: Common

Disease or Pathogen: Foot-and-Mouth Disease

Target: Antibody

Test Type: Elisa Kit

Foot and mouth disease virus antibody

Foot-and-mouth disease (FMD) is a highly contagious viral infection primarily of cloven hoofed domestic animals, such as cattle, pigs, sheep, goats, deer, and water buffalo. In many countries the disease is controlled by vaccinations that consist of (partly) purified structural proteins (SP) of the FMD virus, and therefore vaccinated animals only elicit antibodies directed against the structural proteins. Non structural protein (NSP) is expressed only by replicating viruses, and inactivated vaccines are purified to remove the

cellular proteins and NSP. Therefore, only animals that have been infected with wild type develop antibodies against NSP. It is important to differentiate SP and NSP antibodies in countries that use vaccination to control FMDV outbreaks to discriminate wild type infections and immune response to vaccination

 Indications:

• Discriminate sera between infection and vaccination
• Diagnosis of FMD in non-vaccinated herds
• Screening for test and slaughter government policy

 Special Features:

• Differential test of FMD infected or vaccinated
• High accuracy equivalent to a world standard ELISA kit
• Easy test procedure: No serum pre-dilution required
• Cost effective: No requirement for an uncoated microplate for serum pre-dilution
• Species: Cattle, Sheep, Goat, Pig
• Specimen: Plasma, Serum
• Reading time: 1 hour and 45 minutes
• Sensitivity: Cattle 93.8%
• Specificity: Cattle 99.9%, Pig 99.9%, Sheep & goat 100%

 Quick Procedure:

1. Prepare FMD NSP antigen coated test plate
2. Add 50 μl of controls and sample to wells
3. Add 50 μl of diluted enzyme conjugate to wells
4. Incubate plate for 90 minutes at 37℃
5. Wash plate 6 times
6. Dispense100 μl of substrate (Ready to use) into each well and incubate for 15 minutes at room temperature
7. Add 100 μl of stopping solution
8. Measure the optical density (OD) at 450 nm with reference wavelength at 620nm
9. PI value=[1-(OD sample/mean OD NC)] x 100