AffiVET® Poultry Newcastle Disease NDV Antigen Rapid Test Kit

Test Principal:

• The AffiVET® Rapid NDV Ag Test Kit utilizes chromatographic immunoassay technology to qualitatively detect Newcastle Disease virus (NDV) antigen in samples from chicken's oropharynx, spleen, or kidney.
• The device features a test (T) line and a control (C) line, where the control line serves as a reference ensuring the test is functioning correctly. The appearance of the control line confirms proper test execution.
• Highly selective antibodies specific to NDV are employed in both capture and detection roles within the assay, enabling accurate detection of NDV antigen in chicken tissues such as oropharynx, spleen, or kidney.

Material Required:

Storage & Stability:

• Store the test kit at 2~30℃. DO NOT FREEZE.
• Do not store the test kit in the direct sunlight.
• The test kit is stable within the expiration date marked on the package label.

Sample collection & Preparation:

1. Use samples from chicken oropharynx, spleen, or kidney for this test to ensure accurate detection of Newcastle Disease virus (NDV) antigen.
2. Collect samples by either swabbing the organ (oropharynx, spleen, or kidney) or preparing 20-50% homogenates of spleen or kidney using PBS. For example, for a 50% homogenate, mix 2g of spleen with 2ml of PBS; for a 20% homogenate, mix 1g of spleen with 4ml of PBS..
3. If the sample cannot be tested immediately, refrigerate it at 2-8°C for up to 24 hours. For longer storage, freeze the sample at or below -20°C in vials with airtight seals to maintain sample integrity.

Test Procedure:

1. Bring all kit components and samples to room temperature before use to ensure optimal performance.
2. Collect samples using a swab for accurate testing.
3. Insert the swab into the assay diluent tube and gently mix it for 10 seconds.
4. Wait for 1 minute to allow the samples to settle down before proceeding with the test.
5. Remove the test device from the foil pouch and place it on a flat, dry surface.
6. Using the disposable dropper provided, extract the supernatant sample from the tube.
7. Apply four (4) drops of the sample into the sample hole on the test device, adding each drop one at a time.
8. Start the timer and observe as the sample flows across the result window. If the sample does not appear after 1 minute, add one more drop of the prepared sample to the sample hole.
9. Interpret test results at 10 minutes. Do not read the results after 20 minutes.

Result Interpretation:

Negative result

This means that there is no NDV antigens in the sample or the level is lower than the test detection limit.

Positive result:

This confirms the presence of the NDV antigens in the sample.

Invalid Result:

Absence of C-Line
Presence of T-Line

Or

Absence of C-Line
Absence of T-Line

This means that the procedure was not applied correctly or that the test is invalid.

Cautions:

• The AffiVET® Rapid NDV Ag Test Kit is specifically designed for detecting Newcastle Disease virus (NDV) antigen in chicken samples and should not be used for other animals.
• To ensure accurate results, handle the test device carefully as it is sensitive to humidity and heat. Perform the test immediately after removing the device from the foil pouch.
• Avoid reusing any components of the test kit as it may lead to inaccurate results.
• Apply the assay diluent vertically as per the instructions provided to maintain the integrity of the test.
• Do not touch the membrane in the result window of the test device to prevent any contamination or interference with the results..
• Always check the expiration date marked on the package label and do not use the test kit beyond this date.
• Do not use the test kit if the pouch is damaged or the seal is broken, as this may compromise the accuracy of the results.
• It's important not to mix components from different lot numbers as each kit undergoes quality control testing as a standard batch unit.
• Handle all samples as potentially infectious and wear protective gloves during sample handling. Thoroughly wash hands after handling samples.
• Follow national and local guidelines for the safe decontamination and disposal of all samples, used kits, and any materials that might be contaminated to ensure environmental safety and health protection.