AffiVET® Poultry Infectious Bursal Disease Virus IBDV Antigen Rapid Test
What is Bursal Disease Virus?
Infectious bursal disease (IBD) is seen in young domestic chickens worldwide and is caused by infectious bursal disease virus (IBDV). Signs can include depression, watery diarrhea, ruffled feathers, and dehydration. Morbidity is high and mortality is usually low, but some very virulent strains are capable of causing 60% or higher mortality. Macroscopic and microscopic lesions in the cloacal bursa and molecular identification of the viral genome are used to diagnosis the disease. Vaccination to induce maternal immunity in young chicks is initially used to control the disease. Vectored and live-attenuated vaccines can be used to induce active immunity in chicks as the maternal antibodies wane.
What are symptoms of Bursal Disease virus?
Symptoms of the clinical disease can include depression, watery diarrhea, ruffled feathers, and dehydration. Depending on the IBDV strain and presence of maternal immunity, the disease can also present as a clinical or subclinical disease in young chicks.
Infectious Bursal Disease virus antigen
Infectious Bursal Disease (IBD), or Gumboro Disease, is a viral disease usually affecting young chickens 3 to 6 weeks old, and transmitted by contaminated feed and water. Bursa of Fabricious is the main target organ of IBDV, which is an important organ for young chickens as an immune development. IBDV serotype 1 causes clinical disease in chickens younger than 10 weeks, with older chickens usually showing no clinical signs. IBDV serotype 2 is widespread in turkeys and is sometimes found in chickens and ducks. In practice, a diagnosis can be indicated by the sudden onset of mortality in chickens between 2 and 8 weeks of age, and the presence of distinctive lesions in the Bursa of Fabricious and accompanying blood spots in the musculature of the breast and thigh of affected chickens.
Indications
Field monitoring of Infectious Bursal Disease virus • Tentative diagnosis for swift control in outbreak suspected situation • Differential diagnosis of other avian major diseases
Special Features
Detection of all IBDV • No cross-reaction with other avian viruses • Specimen: Bursa of Fabricious, Cloaca • World's first commercialized rapid test kit for IBDV • Sensitivity: 99.9% vs. RT-PCR • Specificity: 96.6% vs. RT-PCR
Sensitivity and Specificity tests
- Sensitivity : 99.9%
- Specificity : 96.6%
Test Procedures
- Collect swab sample from Bursa of Fabricious or cloaca (feces)
- Insert the swab into the sample tube containing assay diluent and mix the swab until the sample has been dissolved into the assay diluent.
- Take the supernatant with disposable dropper provided.
- Add 4 drops into the sample hole with disposable dropper.
Interpretation
- Negative: Dark Control Line (C) and No Test Line (T)
- Positive: Dark Control Line (C) and Dark Test Line (T)
- Invalid: No Control Line (C) and No Test Line (T)
- Invalid: No Control Line (C) and Dark Test Line (T)