AffiVET® Canine Parvovirus CPV Antigen Rapid Test Kit

Test Principal:

The AffiVET® Rapid CPV Ag Test Kit is a chromatographic immunoassay designed for the qualitative detection of Canine Parvovirus antigen in canine fecal samples.

This kit features two distinct lines on the device's surface: the test (T) line and the control (C) line. Initially invisible in the result window, these lines become visible upon the application of a sample. The control line serves as a crucial reference, confirming the proper execution of the test and is expected to appear with every test conducted.

The presence of CPV antigens in the sample leads to the emergence of a purple test line within the result window.

To ensure high sensitivity and specificity, the assay employs highly selective antibodies against CPV, functioning both as capture and detector elements. These antibodies are adept at identifying CPV antigens in canine feces, thereby ensuring the assay's high accuracy.

Material Required:

Storage & Stability:

1. For this test, only canine whole blood, serum, or plasma should be used.

Whole blood

• Collect blood in an anticoagulant tube. If not tested immediately, refrigerate at 2-8°C and use within 24 hours.

Serum

• Collect blood in a tube without anticoagulants (e.g., heparin, EDTA, sodium citrate).
• Let it settle for 30 minutes to clot, then centrifuge to obtain serum.

Plasma

• Collect blood in a tube with anticoagulants, then centrifuge to obtain plasma.

2. If serum or plasma samples are not tested immediately, refrigerate at 2-8°C. For longer storage, they can be stored frozen at -20°C. Before use, thaw frozen samples to room temperature (15-30°C).

3. Samples with precipitates may give unreliable results. Clarify such samples before testing.

4. Avoid using samples that are hemolytic or contaminated with bacteria, as they may cause incorrect results.

Sample collection & Preparation:

1. Utilize a canine feces swab for this test.
2. Test samples immediately after collection.
3. If immediate testing isn't possible, store samples at 2 to 8°C for 24 hours. For longer storage, freeze at -20°C or below. Prior to use, thaw frozen samples to room temperature (15 to 30°C).
4. Ensure the correct amount of fecal material on the swab as depicted in the accompanying image. Excessive fecal matter may lead to false positive results and hinder migration.

Test Procedure:

1. Ensure all reagents and samples are brought to room temperature (15~30°C) before use.
2. Collect fecal samples using a swab.
3. Insert the swab into the assay diluent tube and thoroughly mix until the sample is dissolved into the diluent (approximately 10 seconds).
4. Allow the mixture to settle for 1 minute to allow large particles to precipitate.
5. Remove the test device from the foil pouch and place it on a flat, dry surface.
6. Using the disposable dropper, transfer the supernatant sample from the tube.
7. Add 4 drops of the mixed sample into the sample hole(s), dropping vertically.
8. Begin the timer and allow the sample to flow across the result window. If no flow is observed after 1 minute, add one additional drop of the mixed sample to the sample hole.
9. Interpret test results at 10 minutes. Do not read the results after 20 minutes.

Result Interpretation:

Negative result

This means that there is no CPV antigens in the sample or the level is lower than the test detection limit.

Positive result:

This confirms the presence of the CPV antigens in the sample.

Invalid Result:

Absence of C-Line
Presence of T-Line

Or

Absence of C-Line
Absence of T-Line

This means that the procedure was not applied correctly or that the test is invalid.

Cautions:

• This test kit is exclusively designed for use in dogs. It is not suitable for testing other animals.
• The test device is sensitive to both humidity and heat. To ensure accuracy, initiate the test promptly after removing the device from its protective foil pouch.
• The components of this test kit are single-use only and should not be reused under any circumstances.
• When applying the sample, use the provided disposable dropper and hold it vertically to ensure precision.
• Avoid making contact with the membrane located in the result window of the test device to prevent contamination or damage.
• Do not use the test kit after its expiration date, which is clearly indicated on the package label, to guarantee the reliability of results.
• If the pouch is damaged or the seal has been tampered with, do not use the test kit as its sterility and integrity may be compromised.
• Refrain from interchanging components between kits of different lot numbers. Each kit's components have undergone quality control testing as a complete unit to ensure their performance.
• Treat all samples as potentially infectious. Protective gloves should be worn during sample handling, and hands should be washed thoroughly afterward.
• Follow national and local guidelines for the safe decontamination and disposal of all samples, used kits, and any materials that might be contaminated.