SKU: AFG-VZ-R423-01

AffiPCR® RT SuperMix for qPCR (+gDNA wiper) V4

Vendor AffiGEN
Regular price €652,00 EUR
Sale price €652,00 EUR Regular price
Sale Sold out
Unit price
Shipping calculated at checkout.
This is a pre order item. We will ship it when it comes in stock.

AffiPCR® RT SuperMix for qPCR (+gDNA wiper) V4

AffiPCR® RT SuperMix for qPCR (+gDNA wiper) V4 is an upgraded version of HiScript III RT SuperMix for qPCR (+gDNA wiper), including a new generation of HiScript IV Reverse Transcriptase and Buffer optimized for reverse transcription. This kit further improves the synthesis efficiency of cDNA, making it a better choice for reverse transcription of low-input, low-expression or degraded RNA templates. 5X gDNA wiper Mix can completely remove the genomic contaimination in the RNA template, so there is no need to design intron-spanning qPCR primers. 4X HiScript IV qRT SuperMix contains all the components required for the reverse transcription, just add template RNA and RNase-free ddH2O to start the reaction.


Components Quantity
RNase-free ddH2O 2 × 1 mL
5X gDNA wiper Mix 300 µL
4X HiScript IV qRT SuperMixa 500 µL
4X No RT Control Mix  50 µL



It is applicable for reverse transcription reactions of animal, plant and microbial RNA. The obtained cDNA is compatible with dye-based and probe-based qPCR.

Storage Conditions:

Store at temperatures between -30°C and -15°C, and ensure transportation at ≤0°C. After thawing, they can be stored at 4°C for up to three months, maintaining their quality and efficacy.