AffiDETECT® Annexin V-EGFP/PI Apoptosis Detection Kit Annexin V-EGFP/PI

Vendor AffiGEN
Regular price €239,88 EUR
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Annexin V-EGFP/PI cell apoptosis detection kit uses EGFP-labeled Annexin V as a probe to detect the occurrence of early cell apoptosis. The detection principle is as follows: in normal living cells, phosphotidylserine (PS) is located on the inner side of the cell membrane, but in early apoptotic cells, PS flips from the inner side of the cell membrane to the surface of the cell membrane and is exposed to the extracellular environment. Annexin-V is a Ca2+ -dependent phospholipid-binding protein with a molecular weight of 35 to 36 kDa that binds to PS with high affinity. The phosphatidyl serine can bind to the membrane of early apoptotic cells through the exposed phosphatidyl serine on the outside of the cell. In addition, Propidium Iodide (PI) is provided to distinguish surviving early cells from necrotic or late apoptotic cells. PI is a nucleic acid dye, which can not pass through the intact cell membrane of normal cells or early apoptotic cells but can pass through the cell membrane of late apoptotic and necrotic cells and make the nucleus red. Thus, when Annexin V was combined with PI, PI was excluded from living cells (Annexin V-/PI-) and early apoptotic cells (Annexin V+/PI-) . The late apoptotic and necrotic cells were double positive for both EGFP and PI (Annexin V+/PI+) . This kit is suitable for flow cytometry and fluorescence microscopy.

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