SKU: AFG-BV-0470

AffiASSAY® MTS Cell Proliferation Colorimetric Assay Kit

Vendor AffiGEN
Regular price €425,00 EUR
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AffiASSAY® MTS Cell Proliferation Colorimetric Assay Kit

AffiASSAY® MTS Cell Proliferation Colorimetric Assay Kit is designed to provide accurate and reliable measurements of cell proliferation in various biological samples. This assay kit offers a robust tool for researchers in the fields of cell biology, pharmacology, and drug discovery.

Key Features:

  • High Sensitivity: Our assay kit is optimized to detect subtle changes in cell proliferation, enabling precise quantification across a wide range of conditions.
  • Colorimetric Detection: Leveraging the colorimetric assay principle, our kit ensures reproducible results with minimal background interference, enhancing assay reliability.
  • Versatile Application: The AffiASSAY® MTS Cell Proliferation Colorimetric Assay Kit is compatible with diverse cell types and culture conditions, facilitating broad experimental applicability.
  • Straightforward Protocol: With a user-friendly protocol, you can obtain proliferation data efficiently, saving time and resources in your research endeavors.
  • Reliable Performance: Whether assessing compound cytotoxicity, screening for proliferation modulators, or studying cellular responses, our kit delivers consistent and dependable results.

Assay Principle:

This assay kit utilizes the reduction of the yellow tetrazolium salt MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) to formazan by metabolically active cells. The formazan product's absorbance is proportional to the number of viable proliferating cells in the sample, allowing accurate quantification of cell proliferation.


  • Cell Viability Assessment: Evaluate the viability and proliferation potential of cultured cells under different experimental conditions.
  • Drug Screening: Screen compounds for their effects on cell proliferation, aiding in the identification of potential therapeutic agents.
  • Proliferation Assays: Study cellular proliferation dynamics in response to stimuli, treatments, or genetic manipulations with precision.
  • Cellular Toxicity Testing: Assess the cytotoxicity of chemicals, drugs, or environmental factors on cell growth and proliferation.

Kit Contents:

  • MTS Reagent
  • Cell Culture Medium
  • Stop Solution
  • 96-Well Assay Plate
  • Detailed Protocol

Why Choose AffiASSAY®?

Our dedication to scientific excellence and quality assurance makes AffiASSAY® the preferred choice for researchers worldwide. We are committed to providing innovative solutions that empower your research and accelerate scientific discoveries.

AffiASSAY® MTS Cell Proliferation Colorimetric Assay Kit Protocol:

[Please note that this protocol serves as a general guideline. Always refer to the specific instructions provided in the kit manual for precise details.]

Materials Needed:

  • AffiASSAY® MTS Cell Proliferation Colorimetric Assay Kit
  • Cultured cells or tissue samples
  • Cell culture plates or vessels
  • 37°C CO2 incubator
  • Microplate reader capable of measuring absorbance at ~490 nm
  • Pipettes and tips


Cell Preparation:

  • Culture cells according to standard protocols until reaching the desired confluence or experimental stage.
  • Harvest cells using trypsin or a suitable cell dissociation reagent and resuspend them in fresh culture medium.
  • Adjust cell density as needed for the assay.

Assay Plate Setup:

  • Seed cells in a 96-well plate at appropriate densities, ensuring uniform distribution across wells.
  • Include wells for blank controls (medium only) and positive controls (untreated cells) as references.

MTS Assay:

  • Prepare the MTS reagent according to the kit instructions.
  • Add an appropriate volume of MTS reagent to each well containing cells.
  • Incubate the plate at 37°C in a CO2 incubator for a specified duration, typically 1-4 hours.
  • After incubation, stop the reaction by adding the provided stop solution.

Optical Density Measurement:

  • Measure the absorbance of the formazan product at around 490 nm using a microplate reader.

Data Analysis:

  • Calculate cell proliferation or viability based on the absorbance readings, comparing experimental wells to controls.
  • Plot data and analyze results according to your research objectives.

Results Interpretation:

  • Interpret assay results in the context of your experimental conditions and hypotheses.
  • Consider factors such as treatment effects, time course, and replicates in result interpretation.

Data Presentation:

  • Prepare figures or tables summarizing your findings for presentation or publication.
  • Include statistical analyses if applicable to enhance result credibility.


  • Dispose of all waste materials properly, following institutional guidelines for biohazardous waste.
  • Ensure proper storage and labeling of remaining kit components for future use.