SKU: AFG-BV-0698

AffiASSAY® L-Lactate Colorimetric Assay Kit

Vendor AffiGEN
Regular price €374,00 EUR
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AffiASSAY® L-Lactate Colorimetric Assay Kit

The AffiASSAY® L-Lactate Colorimetric Assay Kit provides a reliable method for the quantification of L-lactate levels in various biological samples. L-lactate is a key metabolite involved in cellular energy metabolism and is a critical marker in assessing lactate production and clearance in physiological and pathological conditions. This assay kit offers a sensitive colorimetric detection method, enabling researchers to accurately measure L-lactate concentrations with ease.

Key Features:

  • High Sensitivity: The assay kit can detect low levels of L-lactate, ensuring accurate quantification in biological samples with minimal interference.
  • Wide Application: Compatible with various sample types, including serum, plasma, tissue homogenates, and cell lysates, the AffiASSAY® L-Lactate Colorimetric Assay Kit offers versatility in L-lactate-related research.
  • Quick and Easy: With a streamlined protocol, you can obtain results rapidly, saving time and resources in the laboratory.

Assay Principle:

The assay kit measures L-lactate levels based on its enzymatic conversion to generate a product that reacts with a probe to produce a colorimetric signal. The intensity of the signal is directly proportional to the concentration of L-lactate in the sample, allowing for precise quantification.


  • Cellular Metabolism: Study L-lactate production and utilization in cellular energy metabolism and aerobic glycolysis.
  • Exercise Physiology: Assess lactate kinetics and clearance during exercise and physical activity.
  • Pathophysiology: Investigate the role of L-lactate in metabolic disorders, such as lactate acidosis, sepsis, and ischemia.

Kit Contents:

  • L-Lactate Assay Buffer
  • L-Lactate Enzyme Mix
  • L-Lactate Probe
  • Microplate (96 wells)
  • Detailed Protocol

Why Choose AffiASSAY®?

Our commitment to excellence and scientific rigor makes AffiASSAY® the trusted choice of researchers worldwide. We strive to provide you with innovative tools that deliver precise results and accelerate your research efforts.

AffiASSAY® L-Lactate Colorimetric Assay Kit Protocol:

[Please note that this protocol is a general guideline, and you should always refer to the specific instructions provided in the kit manual for accurate and detailed information.]

Materials Needed:

  • AffiASSAY® L-Lactate Colorimetric Assay Kit
  • Biological samples containing L-lactate
  • Microplate reader capable of measuring absorbance
  • Pipettes and tips
  • Microcentrifuge tubes


Standard Curve Preparation:

  • Prepare a series of standard solutions containing known concentrations of L-lactate.
  • Dilute the L-Lactate Assay Buffer and L-Lactate Enzyme Mix as instructed in the kit manual.
  • Add the standard solutions to each well of the microplate to generate the standard curve.

Assay Plate Setup:

  • Label the microplate wells according to your experimental design, including wells for your samples, standards, and controls.
  • If using controls, prepare control samples without L-lactate to serve as background controls.

Sample and Standard Addition:

  • Add 50-100 µL of your prepared samples and standard solutions to the appropriate wells on the microplate.
  • Ensure that the final reaction volume in each well is consistent.

Assay Reaction:

  • Prepare the reaction mixture by combining the L-Lactate Assay Buffer and L-Lactate Enzyme Mix.
  • Add the reaction mixture to each well of the microplate containing the samples and standards.
  • Incubate the microplate at the appropriate temperature (as specified in the kit instructions) for a recommended time to allow the enzymatic conversion of L-lactate.

Colorimetric Measurement:

  • After the incubation period, measure the absorbance at the appropriate wavelength using a microplate reader.

Data Analysis:

  • Calculate the L-lactate levels in your samples based on the absorbance readings and the standard curve generated from the standard solutions.

Results Interpretation:

  • Interpret and analyze your results according to your research objectives.
  • Observe any variations or trends in L-lactate levels among your samples.

Data Presentation:

  • Prepare your data for presentation or publication, and include the appropriate statistical analysis if required.


  • Dispose of all waste materials, including used assay components, according to your laboratory's safety protocols.
  • Always refer to the specific instructions provided with the AffiASSAY® L-Lactate Colorimetric Assay Kit for any kit-specific details or variations in the protocol.
  • Additionally, ensure that you adhere to the best practices and safety guidelines in your laboratory while conducting the assay.