SKU: AFG-BV-0701

AffiASSAY® Glycogen Colorimetric Assay Kit V2

Vendor AffiGEN
Regular price €345,00 EUR
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AffiASSAY® Glycogen Colorimetric Assay Kit V2

The AffiASSAY® Glycogen Colorimetric Assay Kit V2 provides a reliable method for the quantification of glycogen content in various biological samples. This assay kit offers a colorimetric detection method, allowing researchers to study glycogen metabolism, glycogen storage disorders, and carbohydrate metabolism with precision and convenience.

Key Features:

  • High Sensitivity: The assay kit can detect low levels of glycogen, ensuring accurate quantification in biological samples.
  • Wide Application: Compatible with various sample types, including tissues, cells, and biological fluids, the AffiASSAY® Glycogen Colorimetric Assay Kit V2 offers versatility in glycogen analysis.
  • Quick and Easy: With a streamlined protocol, you can obtain results rapidly, saving time and resources in the laboratory.
  • Cost-Effective: Our kit provides an affordable solution for measuring glycogen content, reducing experimental costs without compromising data quality.

Assay Principle:

The assay kit measures glycogen content based on the hydrolysis of glycogen to glucose by glycogenase, followed by the enzymatic oxidation of glucose to generate a colorimetric signal. The signal intensity is directly proportional to the glycogen concentration in the sample.


  • Metabolism Studies: Investigate glycogen metabolism in various tissues and cell types under different physiological and pathological conditions.
  • Disease Research: Study glycogen storage disorders and their underlying mechanisms using reliable glycogen quantification methods.
  • Drug Screening: Screen for compounds that modulate glycogen metabolism as potential therapeutics for metabolic disorders.

Kit Contents:

  • Glycogen Assay Buffer
  • Glycogenase Enzyme
  • Glucose Assay Reagent
  • Glucose Standard
  • Microplate (96 wells)
  • Detailed Protocol

Why Choose AffiASSAY®?

Our commitment to excellence and scientific rigor makes AffiASSAY® the trusted choice of researchers worldwide. We strive to provide you with innovative tools that deliver precise results and accelerate your research efforts.

AffiASSAY® Glycogen Colorimetric Assay Kit V2 Protocol:

[Please note that this protocol is a general guideline, and you should always refer to the specific instructions provided in the kit manual for accurate and detailed information.]

Materials Needed:

  • AffiASSAY® Glycogen Colorimetric Assay Kit V2
  • Biological samples containing glycogen
  • Microplate reader capable of measuring absorbance
  • Pipettes and tips
  • Microcentrifuge tubes


Standard Curve Preparation:

  • Prepare a series of standard solutions containing known concentrations of the glucose standard provided in the kit.
  • Dilute the Glycogen Assay Buffer and Glycogenase Enzyme as instructed in the kit manual.
  • Add the standard solutions to each well of the microplate to generate the standard curve.

Assay Plate Setup:

  • Label the microplate wells according to your experimental design, including wells for your samples, standards, and controls.
  • If using controls, prepare control samples without glycogen to serve as background controls.

Sample and Standard Addition:

  • Add 50-100 µL of your prepared samples and standard solutions to the appropriate wells on the microplate.
  • Ensure that the final reaction volume in each well is consistent.

Assay Reaction:

  • Prepare the reaction mixture by combining the Glycogen Assay Buffer and Glycogenase Enzyme.
  • Add the reaction mixture to each well of the microplate containing the samples and standards.
  • Incubate the microplate at the appropriate temperature (as specified in the kit instructions) for a recommended time to allow the enzymatic reaction to proceed.

Colorimetric Measurement:

  • After the incubation period, measure the absorbance at the appropriate wavelength using a microplate reader.

Data Analysis:

  • Calculate the glycogen content in your samples based on the absorbance readings and standard curve generated from the standard solutions.

Results Interpretation:

  • Interpret and analyze your results according to your research objectives.
  • Observe any variations or trends in glycogen content among your samples.

Data Presentation:

  • Prepare your data for presentation or publication, and include the appropriate statistical analysis if required.


  • Dispose of all waste materials, including used assay components, according to your laboratory's safety protocols.
  • Always refer to the specific instructions provided with the AffiASSAY® Glycogen Colorimetric Assay Kit V2 for any kit-specific details or variations in the protocol.
  • Additionally, ensure that you adhere to the best practices and safety guidelines in your laboratory while conducting the assay.