SKU: AFG-BV-0101

AffiASSAY® Branched Chain Amino Acid (Leu/Ile/Val) Colorimetric Assay Kit

Vendor AffiGEN
Regular price €395,00 EUR
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AffiASSAY® Branched Chain Amino Acid (Leu/Ile/Val) Colorimetric Assay Kit

AffiASSAY® Branched Chain Amino Acid (Leu/Ile/Val) Colorimetric Assay Kit is designed to provide precise and reliable measurements of branched-chain amino acids (BCAAs) in biological samples. This assay kit offers a colorimetric detection method, making it a valuable tool for researchers in the fields of nutrition, metabolism, and clinical biochemistry.

Key Features:

  • High Sensitivity: Our assay kit is optimized to detect even low levels of BCAAs, allowing for accurate quantification in a wide range of samples.
  • Colorimetric Detection: Utilizing a highly sensitive colorimetric assay, our kit ensures robust and reproducible results, minimizing the margin for error.
  • Wide Application: The AffiASSAY® Branched Chain Amino Acid Colorimetric Assay Kit can be used with various sample types, including serum, plasma, cell lysates, tissue homogenates, and culture supernatants.
  • Quick and Easy: With a simple and streamlined protocol, you can obtain results in just a few easy steps, saving you valuable time in the lab.
  • Versatile: Whether you're studying amino acid metabolism, assessing nutritional status, or investigating the role of BCAAs in disease states, our kit provides the versatility you need.

Assay Principle:

This assay kit employs a colorimetric method based on the enzymatic conversion of BCAAs into a product that reacts with a chromogenic reagent to produce a colored compound. The intensity of the color is directly proportional to the concentration of BCAAs in the sample, allowing for precise quantification.


  • Nutritional Studies: Measure BCAA levels in serum or plasma samples to assess dietary intake, protein metabolism, and nutritional status.
  • Metabolic Research: Investigate the role of BCAAs in energy metabolism, protein synthesis, and muscle function.
  • Clinical Biochemistry: Monitor changes in BCAA levels associated with metabolic disorders, liver diseases, and muscle wasting conditions.

Kit Contents:

  • Assay Buffer
  • BCAA Enzyme Mix
  • Chromogenic Reagent
  • 96-Well Assay Plate
  • Detailed Protocol

Why Choose AffiASSAY®?

Our commitment to excellence and scientific rigor makes AffiASSAY® the trusted choice of researchers worldwide. We strive to provide you with innovative tools that deliver precise results and accelerate your research efforts.

AffiASSAY® Branched Chain Amino Acid Colorimetric Assay Kit Protocol:

[Please note that this protocol is a general guideline, and you should always refer to the specific instructions provided in the kit manual for accurate and detailed information.]

Materials Needed:

  • AffiASSAY® Branched Chain Amino Acid Colorimetric Assay Kit
  • Biological samples containing BCAAs
  • 96-well microplate
  • Microplate reader capable of measuring absorbance at specific wavelengths
  • Pipettes and tips
  • Microcentrifuge tubes


Sample Preparation:

  • Prepare your biological samples containing BCAAs. These samples can be serum, plasma, cell lysates, tissue homogenates, or culture supernatants.
  • Ensure that your samples are stored and handled according to your specific experimental requirements.

Assay Plate Setup:

  • Label the microplate wells according to your experimental design, including wells for your samples, standards, and controls.
  • If using controls, prepare control samples without BCAAs to serve as background controls.

Sample and Control Addition:

  • Add 50-100 µL of your prepared samples and BCAA standards to the appropriate wells on the microplate.
  • Ensure that the final reaction volume in each well is consistent.

Assay Reaction:

  • Prepare the assay reaction mix by combining the Assay Buffer and BCAA Enzyme Mix from the kit as instructed.
  • Add the assay reaction mix to each well of the microplate containing samples and standards.


  • Incubate the microplate at the appropriate temperature (as specified in the kit instructions) for a recommended time to allow the enzymatic conversion of BCAAs.

Color Development:

  • Add the Chromogenic Reagent to each well to initiate the color development reaction.
  • Incubate the microplate for a specified time to allow the color to develop.


  • Measure the absorbance of each well at a specific wavelength (provided by the kit) using a microplate reader.

Data Analysis:

  • Generate a standard curve using the absorbance values obtained from the BCAA standards.
  • Calculate the BCAA concentrations in your samples based on their respective absorbance readings and the standard curve.

Results Interpretation:

  • Interpret and analyze your results according to your research objectives.
  • Note any variations or trends in BCAA levels among your samples.

Data Presentation:

  • Prepare your data for presentation or publication, and include the appropriate statistical analysis if required.


  • Dispose of all waste materials, including used assay components, according to your laboratory's safety protocols.
  • Always refer to the specific instructions provided with the AffiASSAY® Branched Chain Amino Acid Colorimetric Assay Kit for any kit-specific details or variations in the protocol.
  • Additionally, ensure that you adhere to the best practices and safety guidelines in your laboratory while conducting the assay.