SKU: AFG-BV-0045

AffiASSAY® Alcohol Dehydrogenase Activity Colorimetric Assay Kit

Vendor AffiGEN
Regular price €418,00 EUR
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AffiASSAY® Alcohol Dehydrogenase Activity Colorimetric Assay Kit

AffiASSAY® Alcohol Dehydrogenase Activity Colorimetric Assay Kit is designed to provide precise and reliable measurements of alcohol dehydrogenase (ADH) activity in biological samples. This assay kit offers a colorimetric detection method, making it a valuable tool for researchers in the fields of biochemistry, cell biology, and molecular biology.

Key Features:

  • High Sensitivity: Our assay kit is optimized to detect even low levels of ADH activity, allowing for accurate quantification in a wide range of samples.
  • Colorimetric Detection: Utilizing a highly sensitive colorimetric assay, our kit ensures robust and reproducible results, minimizing the margin for error.
  • Wide Application: The AffiASSAY® Alcohol Dehydrogenase Activity Colorimetric Assay Kit can be used with various sample types, including cell lysates, tissue homogenates, and purified enzyme preparations.
  • Quick and Easy: With a simple and streamlined protocol, you can obtain results in just a few easy steps, saving you valuable time in the lab.
  • Versatile: Whether you're studying alcohol metabolism, investigating the effects of alcohol on cellular pathways, or screening potential inhibitors, our kit provides the versatility you need.

Assay Principle:

This assay kit employs a colorimetric method based on the enzymatic conversion of alcohol into its corresponding aldehyde by ADH, coupled with the reduction of NAD+ to NADH. The increase in absorbance at 450 nm is directly proportional to the ADH activity in your sample, allowing for precise quantification.


  • Alcohol Metabolism Studies: Assess ADH activity as a key enzyme involved in alcohol metabolism and detoxification processes.
  • Drug Screening: Screen potential inhibitors or activators of ADH for their pharmacological effects.
  • Cellular Signaling: Investigate the role of ADH in cellular signaling pathways and its implications in various physiological processes.

Kit Contents:

  • Assay Buffer
  • NAD+ (Nicotinamide Adenine Dinucleotide)
  • Alcohol Substrate
  • ADH Enzyme
  • Positive Control
  • 96-Well Assay Plate
  • Detailed Protocol

Why Choose AffiASSAY®?

Our commitment to excellence and scientific rigor makes AffiASSAY® the trusted choice of researchers worldwide. We strive to provide you with innovative tools that deliver precise results and accelerate your research efforts.

AffiASSAY® Alcohol Dehydrogenase Activity Colorimetric Assay Kit Protocol:

[Please note that this protocol is a general guideline, and you should always refer to the specific instructions provided in the kit manual for accurate and detailed information.]

Materials Needed:

  • AffiASSAY® Alcohol Dehydrogenase Activity Colorimetric Assay Kit
  • Biological samples containing ADH
  • 96-well microplate
  • Microplate reader capable of measuring absorbance at 450 nm
  • Pipettes and tips
  • Microcentrifuge tubes


Sample Preparation:

  • Prepare your biological samples containing ADH. These samples can be cell lysates, tissue homogenates, or purified enzyme preparations.
  • Ensure that your samples are stored and handled according to your specific experimental requirements.

Assay Plate Setup:

  • Label the microplate wells according to your experimental design, including wells for your samples, standards, and controls.
  • If using controls, prepare a positive control sample with known ADH activity.

Assay Reaction:

  • Prepare the assay reaction mix by combining the Assay Buffer, NAD+, Alcohol Substrate, and ADH Enzyme from the kit as instructed.
  • Add 50-100 µL of the assay reaction mix to each well of the microplate.

Sample and Control Addition:

  • Add 50-100 µL of your prepared samples and positive control to the appropriate wells on the microplate.
  • Ensure that the final reaction volume in each well is consistent.


  • Incubate the microplate at the appropriate temperature (as specified in the kit instructions) for a recommended time to allow the enzymatic reaction to occur.

Absorbance Measurement:

  • Using a microplate reader capable of measuring absorbance at 450 nm, measure the absorbance of each well.

Data Analysis:

  • Calculate the ADH activity in your samples based on their respective absorbance readings.
  • Compare the activity levels to your positive control and perform any necessary normalization.

Results Interpretation:

  • Interpret and analyze your results according to your research objectives.
  • Note any variations or trends in ADH activity among your samples.

Data Presentation:

  • Prepare your data for presentation or publication, and include the appropriate statistical analysis if required.


  • Dispose of all waste materials, including used assay components, according to your laboratory's safety protocols.
  • Always refer to the specific instructions provided with the AffiASSAY® Alcohol Dehydrogenase Activity Colorimetric Assay Kit for any kit-specific details or variations in the protocol.
  • Additionally, ensure that you adhere to the best practices and safety guidelines in your laboratory while conducting the assay.