SKU: AFG-VZ-NA210-01

AffiNGS® VAHTS AmpSeq Library Prep Kit V3

Vendor AffiGEN
Regular price €840,00 EUR
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AffiNGS® VAHTS AmpSeq Library Prep Kit V3

AffiNGS® VAHTS AmpSeq Library Prep Kit V3 is based on ultra-multiplex PCR and several core technologies such as end primer digestion, used to construct amplicon libraries. The whole library construction workflow can be finished in one tube.The whole process takes less than 5 , of which the manual operation time is less than 1.5 h. It is compatible with genomic DNA, FFPE DNA, cfDNA, etc, and amount of Input DNA is 1 - 100 ng. This kit is intended for both the Illumina and lon Torrent sequencing platforms. And corresponding adapters (AffiNGS #AFG-VZ-NA111/NA121) are also provided. It is compatible with panels including lon AmpliSeq Séries Panel and AmpliSeq for Illumina Series Panel. as well as the corresponding individually customized panels.

This kit is comprised of multiplex amplification  module, digestion module and ligation module, which has great amplification performance for different input amounts and customized panels, great efficiency of library ligation, and high coverage and high quality library quickly and easily, and get more stabel and reliable sequencing data. All the reagents provided in the kit have undergone rigorous quality control and functional testing. to ensure the optimal stability and repeatability of library construction.


Components AFG-VZ-N210-01 (24 rxns) AFG-VZ-NA210-02 (96 rxns)
4xX VAHTS Multi-PCR Mix 120 µl 480 µl
VAHTS Digest Mix 2 60 µl 240 µl
VAHTS Ligation Enhancer 144 µl 576 µl
VAHTS Ligation Enzyme Mix 2 24 µl 96 µl
VAHTS HIFI Amplification Mix 600 µl 4 x 600 µl
PCR Primer Mix for ION Torrent  120 µl 480 µl
PCR Primer Mix for lllumina  120 µl 480 µl
TE 1 ml 4 x 1 ml



Store at -30 ~ -15°C and transport at ≤0°C.