SKU: AFG-VZ-N417-01

AffiNGS® Ribo-off rRNA Depletion Kit V2 (Bacteria)

Vendor AffiGEN
Regular price €550,00 EUR
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AffiNGS® Ribo-off rRNA Depletion Kit V2 (Bacteria)

The AffiNGS® Ribo-off rRNA Depletion Kit V2 (Bacteria) is designed to remove ribosomal RNA (rRNA) from the total RNA of gram-positive and gram-negative bacteria. This kit is compatible with an initial template input of 0.01 - 1 μg. The total RNA sample undergoes probe hybridization, RNase H digestion, DNase I digestion, and other steps for the rRNAs (including 16S and 23S rRNA) to be depleted while retaining mRNA and other non-coding RNA. This kit is applicable to both intact and partially degraded RNA samples, and the resulting RNA can be used for the analysis of mRNA and non-coding RNA (such as LncRNA). The optimized reaction system enhances the depletion effect and shortens the workflow time.


Components AFG-VZ-N417-01 (12 rxns) AFG-VZ-N417-02 (24 rxns)
rRNA Probe V2 (Bacteria) 24 µl 48 µl
Probe Buffer 36 µl 72 µl
RNase H Buffer 2 36 µl 72 µl
RNase H Mix 24 µl 48 µl
DNase I Buffer 348 µl 696 µl
DNase I 12 µl 24 µl
Nuclease-free ddH2O 1 ml 1 ml

▲The colors of tube caps for each kit component are indicated


Store at -30 ~ -15℃ and transport at ≤0℃.