AffiELISA® DNase I ELISA Kit

SKU: AFG-HZM-142

AffiELISA® DNase I ELISA Kit

AffiGEN
€1.759,20 EUR
€1.759,20 EUR
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AffiELISA® DNase I ELISA Kit 

AffiELISA® DNase I ELISA Kit  is a highly sensitive and specific immunoassay designed for the quantitative detection of DNase I in biological samples. 

This kit provides reliable results, ensuring precise measurement of DNase I activity with excellent specificity, making it ideal for applications requiring stringent monitoring of DNase I levels. Its robust design allows for consistent performance in research, quality control, and manufacturing environments, where accurate detection of residual DNase I is critical for product safety and purity. By offering ease of use and rapid results, this kit enhances workflow efficiency while delivering reliable, reproducible data.

Intended Use: 

AffiELISA® DNase I ELISA Kit  is intended for the quantitative detection of DNase I activity in biological samples.

Kit components:

Components Description 
Microplate wells Pre-coated with a capture antibody.
Capture antibody Coats the microplate wells and binds to the antigen.
Sample The substance containing the antigen to be detected.
Enzyme-labeled detection antibody Binds to the antigen and is labeled with an enzyme .
Tetramethylbenzidine (TMB) substrate Reacts with the enzyme in the detection antibody to produce a blue color.
Peroxidase Enzyme in the detection antibody that catalyzes the reaction with TMB.
Acid stop solution  Converts the blue color produced by TMB to yellow.
Microplate reader Measures the optical density (OD) to quantify antigen concentration.
Standard curve Used to calculate the antigen concentration based on OD measurements.


Materials Required But Not Supplied:

  • Microplate reader capable of reading absorbance at D450/650 nm.
  • Microplate shaker. 

Procedure workflow:

1. Preparation:

  • Prepare all necessary reagents, washing buffer, enzyme-labeled reagents, standards, and samples.

2. Sample and Standard Addition:

  • Add 100 µL of standards and samples to each well.
  • Incubate at 37°C for 60 minutes.

3. First Wash Cycle:

  • Discard the liquid from the wells.
  • Blot the plate dry on absorbent paper.
  • Add 300 µL of wash buffer to each well.
  • Let it stand for 15-30 seconds.
  • Discard the liquid from the wells.
  • Blot the plate dry on absorbent paper.
  • Repeat the wash cycle (addition of wash buffer, standing, discarding, and blotting) 4 times in total.

4. Enzyme Conjugate Addition:

  • Add 100 µL of 1X enzyme conjugate solution to each well.
  • Incubate at 37°C for 60 minutes.

5. Second Wash Cycle:

  • Discard the liquid from the wells.
  • Blot the plate dry on absorbent paper.
  • Add 300 µL of wash buffer to each well.
  • Let it stand for 15-30 seconds.
  • Discard the liquid from the wells.
  • Blot the plate dry on absorbent paper.
  • Repeat the wash cycle (addition of wash buffer, standing, discarding, and blotting) 4 times in total.

6. Substrate Addition and Incubation:

  • Add 100 µL of substrate solution to each well.
  • Incubate at 37°C in the dark for 15 minutes.

7. Stop Reaction and Reading:

  • Add 50 µL of stop solution to each well.
  • Read the absorbance of the samples at OD450/650 nm.