AffiPCR® One Step qRT-PCR Probe 5X Master Mix V3

AffiPCR® One Step qRT-PCR Probe 5X Master Mix V3

AffiPCR® One Step qRT-PCR Probe 5X Master Mix V3 is single-tube RT-qPCR premix that is suitable for singleplex or multiplex qPCR detection using RNA (e.g. RNA virus) as template, with extremely high stability. The reaction can be performed directly after adding templates, primers and probes. Using gene specific primers (GSP), the reverse transcription and qPCR can be finished in one tube, significantly reducing pipetting procedures and the risk of contamination. This kit can prevent the contamination of PCR product. Combining the superior performance of HiScript III Reverse Transcriptase and hot-start Champagne Taq DNA Polymerase, with an optimized buffering system, the detection sensitivity of HiScript III One Step qRT-PCR Probe 5 × Master Mix can reach 0.1 pg of total RNA or less than 10 copies of RNA templates and is suitable for high-specificity detection systems based on fluorescence labelled probes (e.g. TaqMan).

Storage Conditions:

Store at -30°C ~ -15°C and transport at ≤0°C.

Applications:

This product is suitable for detection of various RNA nucleic acids of animals, plants and microorganisms (viruses, etc.).

Notes:

For research use only. Not for use in diagnostic procedures.