AffiVET® Poultry Avian Influenza Virus AIV Antigen Rapid Tests
Test Principal:
The AffiVET® Rapid AIV Ag Test Kit uses sandwich chromatographic immunoassay technology to qualitatively detect avian influenza type A NP antigen in chicken or duck (excluding wild animal) cloaca discharge and tracheal secretions.
The device features a test (T) line and a control (C) line, where the control line serves as a reference ensuring the test is functioning correctly. The appearance of the control line confirms proper test execution.
Highly selective antibodies specific to avian influenza type A NP antigen are used in both capture and detection roles within the assay.
Material Required:
(25) Sample Buffers
(25) Disposable Swabs
(25) Disposable Pipettes
(01) Instruction Manual
Storage & Stability:
- Store the test kit at 2~30℃. DO NOT FREEZE.
- Protect the kit from light and store it in dry environment.
- The test kit is stable within the expiration date marked on the package label.
Sample collection & Preparation:
- Use samples from avian tracheal secretions, cloacal discharge to ensure accurate detection of AIV antigen.
- If the sample cannot be tested immediately, refrigerate it at 2-8°C for up to 24 hours. For longer storage, freeze the sample at -20°C or below. Frozen samples should be brought to room temperature (15~30°C) prior to use.
Test Procedure:
- Bring all kit components and samples to room temperature before use to ensure optimal performance.
- Collect samples from poultry laryngeal tracheal secretions and cloacal discharges.
- Insert the swab into parts with most secretions and gently swipe the swap to make it fully absorb the secretions.
- Insert the prepared swab into the sample buffer tube provided in the kit.
- Drain the swab after stir by squeeze the liquid out against the wall of the tube.
- Remove the test device from the foil pouch and place it on a flat, dry surface.
- Using the disposable pipette provided, extract the supernatant sample from the tube.
- Apply 3 to 4 drops of the sample into the sample hole on the test device, adding each drop one at a time.
- Start the timer and observe as the sample flows across the result window. If the sample does not appear after 1 minute, add one more drop of the prepared sample to the sample hole.
- Interpret test results at 15 minutes. Do not read the results after 20 minutes.
Result Interpretation:
Negative result
This means that there is no AIV antigens in the sample or the level is lower than the test detection limit.
Positive result:
This confirms the presence of the AIV antigens in the sample.
Invalid Result:
Absence of C-Line
Presence of T-Line
Or
Absence of C-Line
Absence of T-Line
This means that the procedure was not applied correctly or that the test is invalid.
Test Precautions:
- The AffiVET® Rapid AIV Ag Test Kit is intended for use with avian samples only, excluding wild animals. Do not use the test for other animals.
- The test device is sensitive to both humidity and heat. Perform the test immediately after removing the test device from the foil pouch.
- Do not reuse any test components.
- Apply the assay diluent vertically.
- Avoid touching the membrane within the result window of the test device.
Do not use the test kit beyond the stated expiration date marked on the package label. - Do not use the test kit if the pouch is damaged or if the seal is broken.
- Do not mix components from different lot numbers. The components in this kit have been quality control tested as a standard batch unit.
- Handle all samples as potentially infectious. Wear protective gloves when handling samples and wash hands thoroughly afterward.
- Decontaminate and dispose of all samples, used kits, and potentially contaminated materials safely in accordance with national and local regulations.
Test Limitations:
- AffiVET® Rapid AIV Ag Test kit is highly accurate in detecting avian influenza virus type A antigen. However, occasional false results may occur.
- Positive result shall be further confirmed by PCR and/or other official methodologies. Diagnosis of Influenza shall be determined by the vet, considering both clinical symptoms and laboratory tests.
- The Color of Line T is related to influenza antigen contained in the sample. If the antigen concentration is at very high level, Line T may appear and then be lighter, in this case, please future dilute the sample solution with DD water and run the test again to see a stable Line T.
- AffiGEN Inc. and its distributors cannot be held liable for any consequences resulting from the misuse or misinterpretation of the test results.