AffiPEG® mPEG-AA, 350
AffiPEG® mPEG-AA, 350 is a mono-functional polyethylene glycol (PEG) derivative designed for bioconjugation and modification of biomolecules. The product is characterized by a molecular weight of 350 Daltons and features a single methoxy group on one end of the PEG chain and an amino acid (AA) on the other end. This unique structure facilitates its use in various biotechnological and pharmaceutical applications.
Applications of AffiPEG® mPEG-AA, 350
Protein and Peptide Modification:
Conjugation: AffiPEG® mPEG-AA, 350 can be used to conjugate PEG chains to proteins or peptides, enhancing their solubility, stability, and bioavailability. The amino acid functional group reacts with specific amino acid residues on the target molecule, forming stable covalent bonds.
Stability Enhancement: The PEGylation process protects proteins and peptides from enzymatic degradation and immunogenic responses, thereby extending their circulatory half-life.
Drug Delivery Systems:
Controlled Release: The PEG moiety in AffiPEG® mPEG-AA, 350 can be utilized to create prodrugs with controlled release properties. By attaching to active pharmaceutical ingredients (APIs), it can improve the pharmacokinetics and biodistribution profiles of drugs.
Targeted Delivery: PEGylation can enhance the targeting capability of drugs to specific tissues or cells, reducing off-target effects and improving therapeutic efficacy.
Biomolecule Immobilization:
Surface Modification: AffiPEG® mPEG-AA, 350 is often used to modify the surfaces of nanoparticles, microparticles, and other delivery systems. The PEG chains create a hydrophilic barrier that minimizes protein adsorption and prevents aggregation.
Microarray Technology: In microarray applications, AffiPEG® mPEG-AA, 350 can be employed to immobilize biomolecules onto surfaces, maintaining their bioactivity and increasing assay sensitivity.
Diagnostics:
Imaging Agents: PEGylation of imaging agents with AffiPEG® mPEG-AA, 350 can improve their solubility and circulation time, leading to better imaging quality and resolution in diagnostic procedures.
Biosensors: The product can be used in the development of biosensors, where PEGylation helps in maintaining the functionality of the sensing elements and reduces non-specific binding.
Tissue Engineering:
Scaffold Functionalization: AffiPEG® mPEG-AA, 350 can be used to functionalize scaffolds in tissue engineering, providing a biocompatible and non-immunogenic surface that promotes cell attachment and proliferation.
Hydrogel Formation: It is also involved in the formation of hydrogels for controlled drug release and tissue regeneration applications, where the PEG chains contribute to the mechanical properties and degradation rate of the hydrogels.
Bioconjugation Techniques:
Crosslinking Agents: AffiPEG® mPEG-AA, 350 acts as a crosslinking agent in bioconjugation techniques, enabling the formation of stable conjugates between different biomolecules, such as antibodies, enzymes, and nucleic acids.
Functionalization of Polymers: It is used in the functionalization of other polymers to create hybrid materials with tailored properties for specific applications in biotechnology and medicine.
Technical Specifications
Molecular Weight: 350 Daltons
Functional Groups: Methoxy (mPEG) on one end, amino acid (AA) on the other end
Solubility: Highly soluble in water and most organic solvents
Purity: High purity with minimal polydispersity index
Handling and Storage
Storage Conditions: Store at -20°C in a dry and inert atmosphere to prevent degradation and contamination.
Handling Precautions: Use personal protective equipment (PPE) such as gloves and safety glasses when handling the product. Avoid inhalation and contact with skin or eyes.
AffiPEG® mPEG-AA, 350 is a versatile and effective PEGylation reagent, providing numerous advantages in the modification and stabilization of biomolecules across a wide range of applications in biotechnology, pharmaceuticals, and diagnostics.