AffiPCR® SYBR qPCR Master Mix (High ROX Premixed)
AffiPCR® SYBR qPCR Master Mix (High ROX Premixed) is a special premix for qPCR assays based on SYBR Green I fluorescence method. The core component, Champagne Taq DNA Polymerase, is a novel antibody-modified hot-start DNA polymerase with high specificity and detection sensitivity. Equipped with the optimized buffer for qPCR and specificity-promoting factors, it is very suitable for highly specific and sensitive qPCR assays. This product is a 2X premix reagent that contains SYBR Green I at an optimal concentration for qPCR. It can obtain a good standard curve in a wide quantitative range, and accurately quantify target genes with good repeatability and high reliability.
Components:
Components | 500 Reactions | 2500 Reactions |
2X SYBR qPCR Master Mix (High ROX Premixed)* | 4 × 1.25 mL | 5 x 4 × 1.25 mL |
Applications:
It is applicable for the amplification and quantification of DNA samples from all species, including genomic DNA, cDNA, plasmid DNA and λDNA.
Applicable Instruments:
This product is premixed with ROX Reference Dye 2 (high concentration) to correct the fluorescence signal error between wells. It is suitable for the following qPCR instruments:
Applied Biosystems 5700, 7000, 7300, 7700, 7900, 7900HT, 7900HT Fast, StepOne, StepOnePlus; and other qPCR instruments that need to add high concentration of ROX Reference Dye.
Storage Conditions:
Store at -30°C ~ -15°C and protect from light. Transport at ≤0°C. After thawing, the Master mix can be stored stably for 6 months at 2°C ~ 8°C and protected from light.
Equivalence:
AFG-VZ-Q311 | AffiGEN AffiPCR® ChamQ SYBR qPCR Master Mix |
AFG-VZ-Q321 | AffiGEN AffiPCR® ChamQ SYBR qPCR Master Mix (Without ROX) |
AFG-VZ-Q331 | AffiGEN AffiPCR® ChamQ SYBR qPCR Master Mix (Low ROX Premixed) |
AFG-VZ-Q341 | AffiGEN AffiPCR® ChamQ SYBR qPCR Master Mix (High ROX Premixed) |
QPS-201 | TOYOBO THUNDERBIRD SYBR ®qPCR Mix |
RR420 Q/A/B | TaKaRa TB Green®Premix Ex Taq™ (Tli RNaseH Plus) |
175-5271 | Bio-Rad SsoAdvancedTM Universal Probes Supermix |
4472908 | ABI SYBR Select Master Mix |