AffiASSAY® Glucose Colorimetric & Fluorometric Assay Kit
The AffiASSAY® Glucose Colorimetric & Fluorometric Assay Kit offers a reliable method for the quantification of glucose in various biological samples. This versatile assay kit provides flexibility with options for both colorimetric and fluorometric detection, making it suitable for a wide range of research applications in metabolism, diabetes, and glucose homeostasis studies.
Key Features:
- Dual Detection: Our assay kit enables the measurement of glucose using both colorimetric and fluorometric methods, offering versatility based on experimental needs.
- High Sensitivity: The kit can detect low levels of glucose, ensuring accurate quantification in biological samples.
- Wide Application: The AffiASSAY® Glucose Assay Kit is compatible with various sample types, including serum, plasma, urine, cell lysates, and culture media.
- Quick and Easy: With a streamlined protocol, you can obtain results rapidly, saving time and resources in the laboratory.
- Cost-Effective: Our kit provides an affordable solution for measuring glucose levels, reducing experimental costs without compromising data quality.
Assay Principle:
The assay kit measures glucose based on the enzymatic oxidation of glucose to generate a colorimetric or fluorometric signal. The signal intensity is directly proportional to the glucose concentration in the sample.
Applications:
- Metabolism Studies: Investigate glucose metabolism and its role in energy production and storage.
- Diabetes Research: Study glucose homeostasis, insulin resistance, and diabetes-related complications.
- Nutritional Studies: Assess the impact of dietary interventions and nutritional supplements on blood glucose levels and metabolic health.
Kit Contents:
- Glucose Assay Buffer
- Glucose Enzyme Mix
- Glucose Probe
- Microplate (96 wells)
- Glucose Standard
- Detailed Protocol
Why Choose AffiASSAY®?
Our commitment to excellence and scientific rigor makes AffiASSAY® the trusted choice of researchers worldwide. We strive to provide you with innovative tools that deliver precise results and accelerate your research efforts.
AffiASSAY® Glucose Colorimetric & Fluorometric Assay Kit Protocol:
[Please note that this protocol is a general guideline, and you should always refer to the specific instructions provided in the kit manual for accurate and detailed information.]
Materials Needed:
- AffiASSAY® Glucose Colorimetric & Fluorometric Assay Kit
- Biological samples containing glucose
- Microplate reader capable of measuring absorbance and fluorescence
- Pipettes and tips
- Microcentrifuge tubes
Protocol:
Standard Curve Preparation:
- Prepare a series of standard solutions containing known concentrations of the glucose standard provided in the kit.
- Dilute the Glucose Probe with Glucose Assay Buffer to prepare the working solution.
- Add the working solution to each standard solution to initiate the reaction.
Assay Plate Setup:
- Label the microplate wells according to your experimental design, including wells for your samples, standards, and controls.
- If using controls, prepare control samples without glucose to serve as background controls.
Sample and Standard Addition:
- Add 50-100 µL of your prepared samples and standard solutions to the appropriate wells on the microplate.
- Ensure that the final reaction volume in each well is consistent.
Assay Reaction:
- Prepare the Glucose Assay Buffer by diluting as instructed in the kit manual.
- Add the Glucose Enzyme Mix and Glucose Probe to each well to initiate the reaction.
- Incubate the microplate at the appropriate temperature (as specified in the kit instructions) for a recommended time to allow the enzymatic reaction to proceed.
Colorimetric and Fluorometric Measurement:
- After the incubation period, measure the absorbance and fluorescence of each well using a microplate reader.
- For colorimetric detection, measure the absorbance at the appropriate wavelength.
- For fluorometric detection, measure the fluorescence intensity using appropriate excitation and emission wavelengths.
Data Analysis:
- Generate standard curves using the absorbance or fluorescence values obtained from the standard solutions.
- Calculate the glucose concentrations in your samples based on their respective absorbance or fluorescence readings and the standard curves.
Results Interpretation:
- Interpret and analyze your results according to your research objectives.
- Observe any variations or trends in glucose levels among your samples.
Data Presentation:
- Prepare your data for presentation or publication, and include the appropriate statistical analysis if required.
Cleanup:
- Dispose of all waste materials, including used assay components, according to your laboratory's safety protocols.
- Always refer to the specific instructions provided with the AffiASSAY® Glucose Colorimetric & Fluorometric Assay Kit for any kit-specific details or variations in the protocol.
- Additionally, ensure that you adhere to the best practices and safety guidelines in your laboratory while conducting the assay.