AffiPCR® 1st Strand cDNA Synthesis Kit (+gDNA wiper)
AffiPCR® 1st Strand cDNA Synthesis Kit (+gDNA wiper) is an upgraded version of HiScript II 1st Strand cDNA Synthesis Kit (+gDNA wiper), including a new generation of reverse transcriptase HiScript III Reverse Transcriptase and Buffer optimized for reverse transcription, further improving the efficiency of 1st strand synthesis. The 5X gDNA wiper Mix in the kit can quickly remove genomic DNA contamination within 2 min, which makes the results more reliable and simplifies the process of qPCR primer design without the need of spanning an exon-exon junction. The kit contains single-component reverse transcription primers Oligo (dT) 20 VN and Random hexamers, which allows users to choose primers flexibly for subsequent experiments as required. The kit can be used to synthesize full-length cDNA (up to 20 kb) for cloning and other downstream experiments, as well as highly uniform cDNA for qPCR.
Components:
| Component |
AFG-VZ-R312-01 50 rxns (20 μl/rxn) |
AFG-VZ-R312-02 100 rxns (20 μl/rxn) |
| RNase-free ddH2O | 1 ml | 1 ml |
| 5X gDNA wiper Mix | 100 µl | 200 µl |
| 10X RT Mixa | 100 µl | 200 µl |
| HiScript III Enzyme Mixb | 100 µl | 200 µl |
| Oligo (dT)20 VN | 50 µl | 100 µl |
| Random hexamers | 50 µl | 100 µl |
Storage:
Store at -30 ~ -15°C and transport at ≤0°C. 10X RT Mix contains high concentration of DTT, which may precipitate at low temperature. Please restore to room temperature, shake gently and mix thoroughly, wait to redissolve the precipitation before use.
Equivalence:
| AFG-VZ-R312 | AffiGEN AffiPCR® 1st Strand cDNA Synthesis Kit (+gDNA wiper) V3 |
| 11766050 | Thermo Fisher SuperScript™ IV VILO™ Master Mix with ezDNase™ Enzyme |
| 18091050 | Thermo Fisher SuperScript™ IV First-Strand Synthesis System |
| M16325 | Thermo Fisher RevertAid™ Master Mix, with DNase I |
| FSK-101 | TOYOBO ReverTra Ace -ɑ-® |
| E3010 | NEB LunaScript™ RT SuperMix Kit |
| RR047 | TaKaRa PrimeScrip™ RT reagent Kit with gDNA Eraser (Perfect Real Time) |
| 1708841 | Bio-Rad iScript™ Reverse Transcription Supermix for RT-qPCR |