AffiGEN® TransfectTM siRNA In Vitro 1000 Plus
Advanced Formulation for Unrivaled Gene Delivery Performance
AffiGEN® TransfectTM siRNA In Vitro 1000 Plus represents a significant leap forward in gene delivery technology. By integrating pre-screened hydrophobic amino acids into a peptide backbone that spans 36 amino acids in length, this reagent leverages our proprietary pH-Dependent Conformational Change (PDCC) technology. This innovation enables the AffiGEN® TransfectTM siRNA In Vitro 1000 Plus to self-assemble during the formation of the transfection complex, establishing it as an exceptionally versatile and potent tool for gene delivery. Demonstrating over 95% silencing efficiency with just 1 nM siRNA across a wide range of mammalian cells, AffiGEN® TransfectTM siRNA In Vitro 1000 Plus sets a new benchmark for efficiency and versatility in gene transfection.
Contents and Specifications:
AffiGEN® TransfectTM siRNA In Vitro 1000 Plus: 1.0 mL, enough for approximately 1000 reactions, with each reaction designed for transfecting 2.5 pmol siRNA in a 24-well plate.
AffiGEN® TransfectTM siRNA In Vitro 1000 Plus Buffer (5X): 8.0 mL, optimized to ensure maximum transfection efficiency, yielding 40 mL of working solution.
Storage Recommendations:
AffiGEN® TransfectTM siRNA In Vitro 1000 Plus: Refrigerate at 4 °C.
AffiGEN® TransfectTM siRNA In Vitro 1000 Plus Buffer (5X): Keep at room temperature (RT).
When stored under the recommended conditions, the product will maintain its stability and effectiveness for 12 months or more.
Key Advantages:
- Exceptional Efficiency: Achieves up to 95% gene silencing with as little as 0.5 pmol of siRNA (equivalent to 1.0 nM siRNA in a 24-well plate), setting a high standard for performance.
- Designed for Challenging Cells: Formulated specifically to transfect mammalian cells that are typically resistant to conventional methods.
- Simplified Protocol: Offers a straightforward, one-tube reaction process, making transfections easier and more convenient than ever.
- Broad Applicability: Excelling across a wide spectrum of mammalian cells, including those that are notoriously hard to transfect, ensuring broad utility in research.
- Minimal Cytotoxicity: Maintains cell viability and health, thanks to its very low cytotoxic profile.
AffiGEN® TransfectTM siRNA In Vitro 1000 Plus is the culmination of advanced research and development, designed to offer researchers unparalleled efficacy, ease of use, and versatility in genetic manipulation. Whether for routine laboratory applications or cutting-edge genetic research, PepMute™ Plus provides a robust solution for achieving high-efficiency DNA and siRNA delivery with minimal impact on cell health.
siRNA In Vitro Transfection Reagents Specifications and Guide Application
| Product | AffiGEN® TransfectTM siRNA In Vitro 1000 | AffiGEN® TransfectTM siRNA In Vitro 2000 | AffiGEN® TransfectTM siRNA In Vitro 3000 | AffiGEN® TransfectTM siRNA In Vitro 4000 | AffiGEN® TransfectTM siRNA In Vitro 1000 Plus |
| Nucleic Acid | siRNA | siRNA, miRNA, DNA | siRNA | siRNA | siRNA |
| Technology | Peptide-based | Non-liposomal | Liposome formulation | Polymer-based | Peptide-based |
| Efficiency | High | High | High | High | High |
| Cell compatibility | Wide | Wide | Wide | Wide | Wide |
| Off-target effects | Low | Low | Low | Low | Low |
| Cytotoxicity | Moderate | Moderate | Low | Very Low | Moderate |
| Package sizes | 1 mL, 5X 1 mL | 1 mL, 5X 1 mL | 1 mL, 5X 1 mL | 1 mL, 5X 1 mL | 1 mL, 5X 1 mL |
| Storage | 4°C, Buffer at RT | 4°C, Buffer at RT | 4°C, Buffer at RT | 4°C, Buffer at RT | 4°C, Buffer at RT |
| Applications | Gene silencing, RNAi | Gene silencing, miRNA delivery, DNA delivery | Gene silencing | Gene silencing | Gene silencing |
| Target Organisms | Mammalian cells | Mammalian cells | Mammalian cells | Mammalian cells | Mammalian cells |
| Cell Types Suitable For | Wide range of cell types | Wide range of cell types, including primary cells, stem cells, and difficult-to-transfect cells | Wide range of cell types | Wide range of cell types, including suspension cells and difficult-to-transfect cells | Wide range of cell types, including hard-to-transfect cells |
| Additional Notes | N/A | Can be used for in vivo applications | N/A | Optimized for siRNA delivery, but can also be used for DNA transfection | Effective at low concentrations |