AffiGEN® TransfectTM siRNA In Vitro 1000
Introducing AffiGEN® TransfectTM siRNA In Vitro 1000, a revolutionary tool for siRNA delivery. This innovative reagent, inspired by the simulation of virus cell-penetrating peptides (CPPs), achieves exceptional results, boasting over 95% silencing efficiency when used with just 1 nM siRNA across a range of mammalian cells.
AffiGEN® TransfectTM siRNA In Vitro 1000 emerged as the standout choice, validated for its remarkable ability to condense and transfect short nucleic acids, including siRNA, miRNA mimics, and DNA oligos (under 100 bp), into a wide spectrum of mammalian cells.
Key Features:
- Unprecedented silencing efficacy at just 1.0 nM siRNA concentration
- Achieves over 95% gene silencing across diverse cell types
- Streamlined one-tube reaction with a straightforward standard protocol
- Compatible with serum and antibiotics for added versatility
- Easily adaptable to High Throughput Screening (HTS) applications
- Remarkably low cytotoxicity ensures cell viability
- Affordability that doesn't compromise quality
Package:
AffiGEN® TransfectTM siRNA In Vitro 1000 (1.0 mL): Suitable for approximately 1,200 reactions when transfecting 0.5~5.0 pmol siRNA or miRNA mimics in a 24-well plate.
AffiGEN® TransfectTM siRNA In Vitro 1000 Buffer (5X): Expertly formulated for maximal transfection efficiency, comes in an 8.0 mL concentrated stock solution that can be used to prepare 40 mL of working solution.
Applications:
- Ideal for siRNA, miRNA mimics, or mRNA transfection
- Enables seamless DNA/siRNA co-transfection
- Perfect for DNA oligo transfection
Storage:
When stored correctly, the product maintains stability for at least 12 months or longer, ensuring long-term usability and reliability.
siRNA In Vitro Transfection Reagents Specifications and Guide Application
| Product | AffiGEN® TransfectTM siRNA In Vitro 1000 | AffiGEN® TransfectTM siRNA In Vitro 2000 | AffiGEN® TransfectTM siRNA In Vitro 3000 | AffiGEN® TransfectTM siRNA In Vitro 4000 | AffiGEN® TransfectTM siRNA In Vitro 1000 Plus |
| Nucleic Acid | siRNA | siRNA, miRNA, DNA | siRNA | siRNA | siRNA |
| Technology | Peptide-based | Non-liposomal | Liposome formulation | Polymer-based | Peptide-based |
| Efficiency | High | High | High | High | High |
| Cell compatibility | Wide | Wide | Wide | Wide | Wide |
| Off-target effects | Low | Low | Low | Low | Low |
| Cytotoxicity | Moderate | Moderate | Low | Very Low | Moderate |
| Package sizes | 1 mL, 5X 1 mL | 1 mL, 5X 1 mL | 1 mL, 5X 1 mL | 1 mL, 5X 1 mL | 1 mL, 5X 1 mL |
| Storage | 4°C, Buffer at RT | 4°C, Buffer at RT | 4°C, Buffer at RT | 4°C, Buffer at RT | 4°C, Buffer at RT |
| Applications | Gene silencing, RNAi | Gene silencing, miRNA delivery, DNA delivery | Gene silencing | Gene silencing | Gene silencing |
| Target Organisms | Mammalian cells | Mammalian cells | Mammalian cells | Mammalian cells | Mammalian cells |
| Cell Types Suitable For | Wide range of cell types | Wide range of cell types, including primary cells, stem cells, and difficult-to-transfect cells | Wide range of cell types | Wide range of cell types, including suspension cells and difficult-to-transfect cells | Wide range of cell types, including hard-to-transfect cells |
| Additional Notes | N/A | Can be used for in vivo applications | N/A | Optimized for siRNA delivery, but can also be used for DNA transfection | Effective at low concentrations |