Laden Sie das Bild in Galerie -Viewer, AffiGEN® Transfect(TM) DNA & siRNA In Vitro 4000 Kit
Laden Sie das Bild in Galerie -Viewer, AffiGEN® Transfect(TM) DNA & siRNA In Vitro 4000 Kit
Laden Sie das Bild in Galerie -Viewer, AffiGEN® Transfect(TM) DNA & siRNA In Vitro 4000 Kit

SKU: AFG-SNG-TR-011

AffiGEN® Transfect(TM) DNA & siRNA In Vitro 4000 Kit

Verkäuferin AffiGEN
Regulärer Preis €303,00 EUR
Verkaufspreis €303,00 EUR Regulärer Preis
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AffiGEN® TransfectTM DNA & siRNA In Vitro 4000 Kit

Product Description

AffiGEN® TransfectTM DNA & siRNA In Vitro 4000 Kit, harnessed from our innovative and proprietary lipid-conjugation technology, incorporating novel fluorinated cationic lipids, showcases substantial advantages over other lipid-based transfection reagents in the market. AffiGEN® TransfectTM DNA & siRNA In Vitro 4000 Kit represents the most potent yet gentle gene delivery solution, aptly suited for a diverse array of applications, including the transfection of plasmid DNA and/or siRNA into most mammalian cell types. When compared to leading products in the market, AffiGEN® TransfectTM DNA & siRNA In Vitro 4000 Kit proves to be not only more cost-effective but consistently delivers superior transfection efficiency with reduced cytotoxicity.

Kit content

 - AffiGEN® TransfectTM DNA & siRNA In Vitro 4000 Kit Reagent, 1.0 ml, sufficient for 1000 DNA transfections and 1000 siRNA transfections (24-well plate).
 - AffiGEN® TransfectTM DNA & siRNA In Vitro 4000 Buffer (5x), 8.0 mL, to prepare a 40 mL working solution (1x).

Features

 - Versatile: Supports DNA transfection, siRNA transfection, and DNA/siRNA co-transfection.
 - Powerful: Achieves exceptional efficiency with adherent cells.
 - Remarkably low toxicity: Enhances cell viability post-transfection.
 - Economical: Enables more transfections with less reagent.
 - User-friendly: Conducted as a one-tube reaction and compatible with serum/antibiotics.
 - Ideal for high-throughput applications.

Storage

Store AffiGEN® TransfectTM DNA & siRNA In Vitro 4000 Kit Reagent at 4°C and AffiGEN® TransfectTM DNA & siRNA In Vitro 4000 Buffer (5x) at room temperature (RT). When stored correctly, the product maintains stability for 12 months or longer.



DNA In Vitro Transfection Reagents Specifications

Product Nucleic Acid Type Delivery Method Toxicity Efficiency Serum Tolerance
AffiGEN® Transfect™ 1000 - Mammalian Cell DNA Calcium phosphate Precipitation High High High
AffiGEN® Transfect™ DNA In Vitro 2000 V1 DNA, siRNA Polymer Polymer complex Low High Low
AffiGEN® Transfect™ DNA In Vitro 2000 V2 DNA, siRNA Polymer Polymer complex Moderate Higher Medium
AffiGEN® Transfect™ DNA In Vitro 2000 Plus DNA Polymer Polymer complex Low Higher Medium
AffiGEN® Transfect™ DNA In Vitro 3000 DNA Liposomal Liposome-mediated Moderate High High
AffiGEN® Transfect™ DNA & siRNA In Vitro 4000 Kit DNA, siRNA Liposomal Liposome-mediated Very low High High
AffiGEN® Transfect™ DNA In Vitro 5000 DNA, siRNA Polymer Polymer complex Moderate High High

 

DNA In Vitro Transfection Reagents Application Guide

Product Applications Cell Types Cell lines Suitable for Additional Notes
AffiGEN® Transfect™ 1000 - Mammalian Cell Protein production, stable cell line generation, viral vector production Adherent, some suspension HEK293, HepG2, CHO Large-scale transfection, cost-effective
Lower calcium concentration than traditional methods
AffiGEN® Transfect™ DNA In Vitro 2000 V1 Gene expression studies, protein production, virus production Most, including sensitive Various Sensitive cell lines, maximizing cell viability
Multiple formulations available
AffiGEN® Transfect™ DNA In Vitro 2000 V2 Gene expression studies, protein production, virus production Most, including sensitive Various Balancing efficiency and viability
Released from DNA complex
AffiGEN® Transfect™ DNA In Vitro 2000 Plus Protein production, viral vector production Most, including sensitive Various Long DNA, high efficiency
Proprietary polymer
AffiGEN® Transfect™ DNA In Vitro 3000 Viral vector production, antibody production, protein production Adherent, suspension Various Lentivirus production, high efficiency
Enhanced conjugate
AffiGEN® Transfect™ DNA & siRNA In Vitro 4000 Kit Protein production, gene editing, virus production Most, including sensitive Various Low toxicity, high efficiency
Fluorinated cationic lipids
AffiGEN® Transfect™ DNA In Vitro 5000 Gene expression studies, protein production, virus production Most, including sensitive Various Common cell lines, biodegradable
Biodegradable polycationic polymer