AffiGEN® T7 High Yield RNA Transcription Kit
The AffiGEN® T7 High Yield Transcription Kit is an optimized system for high yield in vitro transcription of RNA from DNA templates containing T7 RNA Polymerase promoter. The kit contains T7 RNA Polymerase which can synthesize RNA quickly and easily from the downstream of the T7 promoter, and obtains a large amount of RNA. Modified nucleotide can be added to the system to generate biotin or dye-labeled RNA. This kit can yield 150 - 200 μg of RNA with a template input of 0.5 μg. The RNA yield can be widely used in many downstream applications such as RNA structure and function studies, RNase protection, probe hybridization, RNAi, microinjection and in vitro translation.
Storage Conditions:
Store at -30 ~ -15℃ and transport at ≤0℃.
Application:
In vitro transcription of RNA.
Self-prepared Materials:
- Template: linearized plasmid with a T7 RNA polymerase promoter sequence, PCR product or synthetic DNA fragment.
- Purification: phenol, chloroform, sodium acetate, ethanol; or RNA purification column; RNasefree ddH2O.
- Other: RNase-free EP tube, pipette tip; PCR instrument.
Note:
For research use only. Not for use in diagnostic procedures