AffiNGS® Ribo-off rRNA Depletion Kit V2 (Bacteria)
The AffiNGS® Ribo-off rRNA Depletion Kit V2 (Bacteria) is designed to remove ribosomal RNA (rRNA) from the total RNA of gram-positive and gram-negative bacteria. This kit is compatible with an initial template input of 0.01 - 1 μg. The total RNA sample undergoes probe hybridization, RNase H digestion, DNase I digestion, and other steps for the rRNAs (including 16S and 23S rRNA) to be depleted while retaining mRNA and other non-coding RNA. This kit is applicable to both intact and partially degraded RNA samples, and the resulting RNA can be used for the analysis of mRNA and non-coding RNA (such as LncRNA). The optimized reaction system enhances the depletion effect and shortens the workflow time.
Components:
| Components | AFG-M-0471 (12 rxns) | AFG-M-0472 (24 rxns) |
| rRNA Probe V2 (Bacteria) | 24 µl | 48 µl |
| Probe Buffer | 36 µl | 72 µl |
| RNase H Buffer 2 | 36 µl | 72 µl |
| RNase H Mix | 24 µl | 48 µl |
| DNase I Buffer | 348 µl | 696 µl |
| DNase I | 12 µl | 24 µl |
| Nuclease-free ddH2O | 1 ml | 1 ml |
▲The colors of tube caps for each kit component are indicated
Storage:
Store at -30 ~ -15℃ and transport at ≤0℃.