Precast Protein Plus Gel 12%
AffiGEN® Precast Protein Plus Gel 12% - Sharp Bands, Faster Runs, Ready-to-Use Precision
A ready-to-use 12% Hepes-Tris precast protein gel designed for efficient SDS-PAGE workflows, clear protein band separation, and consistent electrophoresis performance.
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12% Gel Format
Suitable for routine protein separation workflows requiring a 12% acrylamide gel.
10 Wells
Designed for practical sample loading in standard protein electrophoresis experiments.
Hepes-Tris Buffering
Supports fast electrophoresis and sharp, clear protein band resolution.
Cold Pack Shipping
Shipped under cold pack conditions to support product stability during transport.
Why Use AffiGEN® Precast Protein Plus Gel?
The AffiGEN® Precast Protein Plus Gel, 12%, 10 wells, Hepes Tris is developed for laboratories that need reliable protein separation without the time-consuming preparation of manual gels. Its ready-to-use format helps simplify SDS-PAGE workflows while supporting clean, sharp, and reproducible protein band patterns.
The Hepes-Tris buffering system is designed to support efficient electrophoresis performance, helping researchers reduce preparation time and improve workflow consistency in routine protein analysis.
Product Information
| Product Name | AffiGEN® Precast Protein Plus Gel, 12%, 10 wells, Hepes Tris |
|---|---|
| Catalog Number | AFG-YSN-387 |
| Gel Percentage | 12% |
| Well Format | 10 wells |
| Buffer System | Hepes-Tris |
| Size | 10 × 10 Piece/Box |
| Shipping Condition | Cold pack |
Recommended Applications
AffiGEN® Precast Protein Plus Gel 12% can be used in routine protein electrophoresis workflows, including SDS-PAGE protein separation, protein purity assessment, protein profiling, molecular weight comparison, and western blot sample preparation.
General SDS-PAGE Protocol for AffiGEN® Precast Protein Plus Gel 12%, 10 Wells
The following workflow provides a general protocol for using AffiGEN® Precast Protein Plus Gel, 12%, 10 wells, Hepes Tris. Always adapt the conditions according to your electrophoresis system, sample type, protein size range, running buffer, and laboratory protocol.
Equilibrate the Gel
Remove the precast gel from cold storage and allow it to equilibrate briefly to room temperature before use. Avoid leaving the gel at room temperature for extended periods.
Prepare the Gel Cassette
Open the gel package carefully. Remove the comb, protective tape, or sealing strip according to the gel cassette format.
Install the Gel in the Electrophoresis Tank
Place the gel cassette into a compatible electrophoresis tank. Make sure the cassette is properly seated to prevent buffer leakage during the run.
Add Running Buffer
Fill the inner and outer chambers with the appropriate running buffer recommended for your electrophoresis system and Hepes-Tris gel workflow.
Prepare Protein Samples
Mix protein samples with suitable SDS-PAGE loading buffer. Heat or reduce samples only when required by your experimental protocol.
Rinse the Wells
Gently rinse the wells with running buffer before sample loading. This helps remove storage solution or residual gel material and supports cleaner migration.
Load Marker and Samples
Load a protein marker and prepared samples into the 10 wells. Avoid overloading the wells to maintain sharp and clear band separation.
Run Electrophoresis
Run the gel using voltage and time conditions suitable for your electrophoresis system. Stop the run when the dye front reaches the desired position.
Process the Gel
Remove the gel cassette carefully, open it, and proceed with staining, imaging, protein transfer, or downstream western blot analysis.
Workflow Advantages
| Ready-to-use format | Reduces manual gel preparation time and supports faster experimental setup. |
|---|---|
| Sharp protein bands | Designed to support clear and defined protein separation patterns. |
| Consistent performance | Manufactured to support reliable batch-to-batch electrophoresis results. |
| Practical 10-well layout | Suitable for routine sample loading, marker comparison, and standard SDS-PAGE workflows. |
FAQ
What is AffiGEN® Precast Protein Plus Gel 12% used for?
It is used for protein electrophoresis workflows such as SDS-PAGE, protein separation, protein profiling, protein purity checks, and western blot preparation.
What is the benefit of the Hepes-Tris buffering system?
The Hepes-Tris buffering system supports efficient electrophoresis, sharp protein bands, and reduced running time compared with traditional manual gel preparation workflows.
How many wells does this gel have?
This AffiGEN® precast protein gel format contains 10 wells.
How do I use AffiGEN® Precast Protein Plus Gel?
Place the gel cassette into a compatible electrophoresis tank, add the appropriate running buffer, prepare and load protein samples, run the gel under suitable electrophoresis conditions, then process the gel for staining, imaging, or western blot transfer.
Should the wells be rinsed before loading samples?
Yes. Rinsing the wells gently with running buffer before sample loading can help remove storage solution and support cleaner sample migration.
Can this gel be used before western blotting?
Yes. This precast protein gel can be used for SDS-PAGE protein separation before transfer to a membrane for western blot analysis.
What is the catalog number?
The catalog number is AFG-YSN-387.
Ready-to-Use Protein Separation with AffiGEN®
Choose AffiGEN® Precast Protein Plus Gel, 12%, 10 wells, Hepes Tris for practical SDS-PAGE workflows, sharp protein bands, and simplified electrophoresis preparation.
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