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Load image into Gallery viewer, AffiVET® Canine Leishmania Antibody Rapid Test Kit
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Load image into Gallery viewer, AffiVET® Canine Leishmania Antibody Rapid Test Kit
Load image into Gallery viewer, AffiVET® Canine Leishmania Antibody Rapid Test Kit

SKU: AFG-VR-33

AffiVET® Canine Leishmania Antibody Rapid Test Kit

Vendor AffiGEN
Regular price €84,00 EUR
Sale price €84,00 EUR Regular price
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AffiVET® Canine Leishmania Antibody Rapid Test Kit

  • Cat Number: AFG-VR-33
  • Size: 10 Tests
  • Species: Canine
  • Sample: Serum/Plasma

Product Datasheet

Test Principle

  • The AffiVET® Canine Leishmania Antibody Rapid Test Kit utilizes rapid immunochromatography technology to detect LSH antibodies. Upon adding the diluted sample to the test card well, any pathogenic antibodies present in the sample will specifically bind to the colloidal gold-labeled antigen. This forms a complex that migrates along the chromatography membrane and is captured on the membrane's pre-coated protein, resulting in a distinctive wine-red detection line at the T position.
  • If the sample does not contain antibodies to the pathogen, no visible line will form at the “T” position. Additionally, a “C” line is incorporated into the system to validate the experiment's effectiveness. Regardless of whether the result is negative or positive, the line should exhibit coloration; otherwise, the result will be deemed invalid.

Materials Required

(10) Canine Leishmania antibody test card bag (contains 1 test card, 1 dropper, 1 bag of desiccant)
(10) Assay Diluents
(10) Droppers
(01) User Manual

Storage & Expiry Date

  • Store the test kit at 2 to 30°C in a cool and dry place. DO NOT FREEZE.
  • Avoid direct Sunlight
  • Expiry date is 18 months from the production date, indicated on the box.

Sample Collection & Preparation

Plasma:

  • Collect blood using anticoagulant tubes.
  • Centrifuge at 1500 rpm for 10 minutes and collect plasma from the top layer.
  • Short-term: Store plasma at 4°C for 2-3 days.
  • Long-term: Freeze plasma at -20°C or lower.

Serum:

  • Collect blood and incubate at 37℃ for 1-2 hours.
  • Centrifuge at 1500r/min for 10 minutes, then collect the serum.
  • Short-term: Store serum at 4℃ for 2-3 days.
  • Long-term: Freeze serum at -20°C.

Test Procedure

  1. Prior to conducting the test, carefully review the instruction manual. Ensure the test card and sample are acclimated to room temperature, ranging between 15°C to 25°C.
  2. Remove a piece of aluminum foil bag and tear it open. Place the test card horizontally on the operating platform without lifting it during the test process. It can only be removed after completion.
  3. Retrieve one bottle of assay buffer from the buffer box. Using a capillary dropper, collect 2 drops (20 μL) of serum or plasma sample. Add the sample to the buffer and thoroughly mix.
  4. Utilize the provided dropper within the aluminum foil bag to draw the mixture. Add 4 drops (100μL) to the sample hole on the test card and commence timing.
  5. Begin interpreting test results after approximately 5 minutes, ensuring completion within 10 minutes. Interpretations beyond 10 minutes are deemed invalid.

Result Interpretation

Negative Result:

  • Presence of C-Line
  • Absence of T-Line

This means there is no C. LSH Ab antibody in sample or the level is lower than the detection threshold.

Positive Result:

  • Presence of both C-Line and T-Line

Purple-red strips confirms the presence of C. LSH Ab antibodies in sample, Regardless of whether the T line is clear or not.

Invalid Result:

  • Absence of C-Line with Presence of T-Line
  • Absence of both C-Line and T-Line

This means that the procedure was not applied correctly or that the test is invalid.

Cautions

  • Use the test card within its shelf life and within one hour of opening.
  • Avoid direct sunlight and airflow during inspection.
  • Handle the test card carefully, avoiding contact with the detection card surface.
  • Prevent cross-contamination.
  • Use only provided sample diluent.
  • Dispose of used test cards properly.

This immunological diagnostic kit is intended solely for providing qualitative test results for the clinical detection of pet diseases. If you have any inquiries regarding the test results, consider employing alternative diagnostic methods, such as enzyme-linked immunoassay kits, for further sample analysis. For pathological analysis, please consult your local veterinary physician.