AffiVET® Bovine & Goat Tuberculosis (TB) Antibody Rapid Test

AffiVET® Bovine & Goat Tuberculosis (MTB) Antibody Rapid Test

Product Insert

Test Principal:

• This test strip uses a rapid immuno-chromatography technique to qualitatively detect Tuberculosis Antibodies in serum and whole blood samples from bovines and goats.
• During testing, the Tuberculosis Antibodies in the sample bind with the antigens coated with the colloidal gold, forming complexes that move towards the other edge of the strip.
• Upon reaching the T-line, the specific antigen on the membrane captures these complexes, resulting in the appearance of a T-line.
• The presence of a C-line indicates the validity of the test.
• If the T-line appears, it signifies the presence of Tuberculosis Antibodies in the sample.

Material Required:

Storage & Expiry Date:

Sample collection & Preparation:

Whole blood:

• Collect fresh anticoagulated whole blood and use on the same day.
• Whole blood without adding anticoagulant should be used immediately after collection.

Serum:

• Collect 2 to 3 mL of blood using a clean, dry tube following standard procedures.
• Allow the blood to last undisturbed for approximately 1 hour until it coagulates.
• After coagulation, centrifuge the tube at 4000 revolutions per minute (r/min) for 10 minutes. Alternatively, you can let the blood sit for about 2 hours until it coagulates and naturally separates into serum and clot.
• Carefully separate the clear and bright serum from any hemolysis or contamination.
• The serum should be stored at a temperature between 2°C to 8°C for short-term storage and at -20°C for long-term storage.

Test Procedure:

• Use dropper to absorb the well-prepared sample.
• Drop 1 drop of serum or 2 drops of whole blood into the sample buffer tube vertically and slowly mix it for ready use. (Note that this step is very important, never add too much sample) 
• Take out the test card from the bag.
• Use the dropper to absorb the sample.
• Drop 2 to 3 drops into the well marked with “S”.
• Read the results in 10 to 15 min at room temperature.
• The result is invalid after 15 min.

Result Interpretation:

Negative Result:

• Presence of C-Line
• Absence of T-Line

This means there is no MTB antibodies in the sample or the level is lower than the detection threshold.

Positive Result:

• Presence of C-Line
• Presence of Dark T-Line

This confirms the presence of the MTB antibodies in the sample. It is a strong positive.

• Presence of C-Line
• Presence of Light T-Line

This confirms the presence of the MTB antibodies in the sample. It is a weak positive.

Invalid Result:

• Absence of C-Line
• Presence of T-Line

or

• Absence of C-Line
• Absence of T-Line

This means that the procedure was not applied correctly or that the test is invalid.

Cautions:

This kit is designed for detecting Tuberculosis antibodies in bovine  and goat sera. Please ensure you understand its intended use completely.

• Read the manual carefully before use. The offered buffer can only be used for this test.
• Avoid using serum from other animal species as a negative control. It is like using "no sample requirement" liquid.
• Do not use product out of date or broken foil bag.
• Return test card and sample buffer to room temperature after taking out from refrigerator, use the test card soon after open package to avoid dampness lead to invalidation.
• Avoid using samples contaminated, with turbidity, severe hemolysis, and large amounts of blood lipids.
• Do not touch the white membrane surface in the middle of test card, avoid sunlight and fan blowing directly.
• The waste of test should be treated as pollution, handle it according to local regulations.