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SKU: AFG-SNG-TR-013

AffiGEN® Transfect(TM) DNA In Vitro 5000

Vendor AffiGEN
Regular price €207,00 EUR
Sale price €207,00 EUR Regular price
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AffiGEN® TransfectTM DNA In Vitro 5000

Product Description

Based on our proprietary polymer synthesis technology, AffiGEN® TransfectTM DNA In Vitro 5000 is formulated as a biodegradable polymer based DNA transfection reagent that ensures effective and reproducible transfection on HEK293, COS-7, NIH-3T3, HeLa, CHO and a broad ranges of hard-to-transfect mammalian cells. The AffiGEN® Transfect 5000 is able to immobilize DNA migration during electrophoresis at very low concentration and form the transfection complex within 5 minutes at RT.

A remarkable feature of the reagent is the rapid and complete degradation of polymer after transfection complex endocytosis , leading to much less cytotoxicity.

The reagent, 1.0 ml, is sufficient for more than 600 transfections in 24 well plates or more than 300 transfections in 6 well plates, providing a very affordable alternative to the leading products for transfecting a variety of commonly used and hard-to-transfect mammalian cells.

Features

- Bio-degradable after endocytosis
- Exceptional high titers of virus production
- Equally good for very long DNAs (>89 kb)
- Equally good for both single DNA transfection and multi DNA co-transfection
- High levels of recombinant protein production
- Simple & robust transfection procedure
- Very affordable

Storage Condition

Store at 4 °C. If stored properly, the product is stable for 12 months or longer.


DNA In Vitro Transfection Reagents Specifications

Product Nucleic Acid Type Delivery Method Toxicity Efficiency Serum Tolerance
AffiGEN® Transfect™ 1000 - Mammalian Cell DNA Calcium phosphate Precipitation High High High
AffiGEN® Transfect™ DNA In Vitro 2000 V1 DNA, siRNA Polymer Polymer complex Low High Low
AffiGEN® Transfect™ DNA In Vitro 2000 V2 DNA, siRNA Polymer Polymer complex Moderate Higher Medium
AffiGEN® Transfect™ DNA In Vitro 2000 Plus DNA Polymer Polymer complex Low Higher Medium
AffiGEN® Transfect™ DNA In Vitro 3000 DNA Liposomal Liposome-mediated Moderate High High
AffiGEN® Transfect™ DNA & siRNA In Vitro 4000 Kit DNA, siRNA Liposomal Liposome-mediated Very low High High
AffiGEN® Transfect™ DNA In Vitro 5000 DNA, siRNA Polymer Polymer complex Moderate High High

 

DNA In Vitro Transfection Reagents Application Guide

Product Applications Cell Types Cell lines Suitable for Additional Notes
AffiGEN® Transfect™ 1000 - Mammalian Cell Protein production, stable cell line generation, viral vector production Adherent, some suspension HEK293, HepG2, CHO Large-scale transfection, cost-effective
Lower calcium concentration than traditional methods
AffiGEN® Transfect™ DNA In Vitro 2000 V1 Gene expression studies, protein production, virus production Most, including sensitive Various Sensitive cell lines, maximizing cell viability
Multiple formulations available
AffiGEN® Transfect™ DNA In Vitro 2000 V2 Gene expression studies, protein production, virus production Most, including sensitive Various Balancing efficiency and viability
Released from DNA complex
AffiGEN® Transfect™ DNA In Vitro 2000 Plus Protein production, viral vector production Most, including sensitive Various Long DNA, high efficiency
Proprietary polymer
AffiGEN® Transfect™ DNA In Vitro 3000 Viral vector production, antibody production, protein production Adherent, suspension Various Lentivirus production, high efficiency
Enhanced conjugate
AffiGEN® Transfect™ DNA & siRNA In Vitro 4000 Kit Protein production, gene editing, virus production Most, including sensitive Various Low toxicity, high efficiency
Fluorinated cationic lipids
AffiGEN® Transfect™ DNA In Vitro 5000 Gene expression studies, protein production, virus production Most, including sensitive Various Common cell lines, biodegradable
Biodegradable polycationic polymer