5 min TA/Blunt-Zero Cloning Kit is a second generation TOPO cloning kit that contains a second generation Topoisomerase, a vector containing the suicide gene ccdB and a blunt end factor. Combining with the optimal buffer, the second generation of Topoisomerase provides a highly efficient, 5 min, one-step cloning strategy at room temperature. This product using a vector containing the suicide gene ccdB, when the insert is successfully ligated to the vector, the correct expression of ccdB is destroyed, and the host cell can grow normally, otherwise the host cell cannot grow normally, thereby achieving “zero” background. Containing a blunt end factor, 5 min TA/Blunt-Zero Cloning Kit is compatible with both TA clones and blunt clones.
Application:
It is suitable for ligation of blunt ended and A-Tailed PCR products
Notes:
For research use only. Not for use in diagnostic procedures,
Components
| Components | 25 Reactions | 50 Reactions |
| 5 × TA/Blunt-Zero Cloning Mixa | 25 μL | 2 X 25 μL |
| 500 bp Control insert (20 ng/μl) | 5 μL | 10 μL |
| M13 Primer Mix (10 μM)b | 200 μL | 400 μL |