AffiCHECK® Eastern Equine Encephalitis (EEE) RNA - PCR Qualitative Positive Control
Product Datasheet - AFG-CHK-0483
PRODUCT DESCRIPTION:
AffiCHECK® Eastern Equine Encephalitis (EEE) RNA - PCR Qualitative Control is a high-quality, reliable standard containing known quantities of Eastern Equine Encephalitis (EEE) RNA. It's designed using modified and non-infectious parasite to ensure stability, thereby contributing to accurate and reliable results in PCR applications.
INTENDED USE:
AffiCHECK® Eastern Equine Encephalitis (EEE) RNA - PCR Qualitative Control serves as a comprehensive process control for the evaluation of the performance of nucleic acid tests for determination of the presence of Eastern Equine Encephalitis (EEE) RNA nucleic acid. It's essential in validating the performance of PCR assays in clinical, research, and diagnostic settings.
PRODUCT FEATURES:
- Specificity: Tailored for Eastern Equine Encephalitis (EEE) RNA detection.
- Formulation: Lyophilized for enhanced stability.
- Convenience: Ready-to-use upon reconstitution.
- Compatibility: Suitable for a wide range of PCR assays.
CHARACTERISTICS:
- Cultivation & Extraction: Cultivated in Vero cells and extracted using a reliable genomic RNA method.
- Quality Control: Subjected to rigorous testing to ensure batch consistency and reliability.
- Packaging: Provided in a protective, heat-sealed foil pouch with silica gel.
PERFORMANCE CHARACTERISTICS:
- Sensitivity: Optimized for high sensitivity in PCR detection.
- Specificity: Exclusively detects Eastern Equine Encephalitis (EEE) RNA.
- Reproducibility: Consistent results across different test runs and batches.
PACK CONTENT :
| Components | Pack Size | Note |
| RNA Control Lyophilized Powder (1.25x104 to 2.00x104 Copies/µL) | 1 Vial | Quantified by Real-Time PCR. |
| Buffer | 500 µL | Water, DNase, RNase Free. |
PRECAUTIONS:
- For research use or diagnostic control purposes only.
- Follow standard laboratory practices and use PPE.
- Use Sterile Filter Tips to prevent contamination.
- Handle infectious specimens with precautions.
- Disinfect work surfaces.
- Maintain separate working areas for testing.
- Dispose of unused reagents and waste as per regulations.
- May contain genetic material, handle with care.
- No method ensures total absence of infectious agents.
- Follow local waste disposal rules.
- Dilute below 103 copies/µL immediately before use.
- Avoid freezing for low-copy dilutions to prevent RNA degradation.
INSTRUCTIONS FOR USE:
- Open the foil pouch of AffiCHECK® Eastern Equine Encephalitis (EEE) RNA.
- Centrifuge AffiCHECK® Eastern Equine Encephalitis (EEE) RNA Lyophilized Powder at 1000 g for 1 minute.
- Add 100 μL of the Buffer and thoroughly mix until fully reconstituted.
- The concentration will be 1.25x104 to 2.00x104 Copies/µL once reconstituted.
- Vortex for 1 min for complete dissolution and homogenization.
RECOMMENDED STORAGE:
- Before Reconstitution: Store at 2-8ºC.
- After Reconstitution: Store at -20ºC.
- Shipping: No special instructions
- Shelf Life: 12 months from production date.
ORDERING INFORMATION :
| Catalog Number | Description | Size |
| AFG-CHK-0483 | AffiCHECK® Eastern Equine Encephalitis (EEE) RNA PCR Qualitative Positive Control | 1 vial + 500 µL Buffer |