AffiAB® Goat anti-V5 Polyclonal IgG Antibody
AffiAB® Goat anti-V5 Polyclonal IgG Antibody offers researchers an efficient tool for studying the molecule V5. This affinity-purified IgG antibody provides high specificity and sensitivity, making it reliable and easy to use in various applications.
Host: Goat
Species: N/A
Description:
The AffiAB® Goat anti-V5 Polyclonal IgG Antibody is an antibody derived from goats that specifically targets and binds to the V5 tag. The V5 tag is a short peptide sequence (GKPIPNPLLGLDST) that is often genetically fused to proteins of interest for various applications in molecular biology and protein research.
This polyclonal antibody is generated by immunizing goats with purified V5-tagged proteins, resulting in the production of antibodies that specifically recognize and bind to the V5 tag. The antibody can be used to detect and study the expression, localization, and interaction of V5-tagged proteins.
The AffiAB® Goat anti-V5 Polyclonal IgG Antibody is commonly used in research applications such as Western blotting, immunoprecipitation, and immunofluorescence. It allows researchers to detect and analyze V5-tagged proteins in a variety of experimental settings.
To ensure optimal results, it is important to follow the recommended protocols and dilutions provided by the manufacturer. Proper controls and optimization steps should be included in experiments to ensure specific and reliable detection of V5-tagged proteins.
For detailed instructions on the specific applications and usage of the AffiAB® Goat anti-V5 Polyclonal IgG Antibody, please refer to the product datasheet or consult the manufacturer's instructions.
Gene Identifier/Accession Number: N/A
Form: N/A
Concentration: 2.5 mg/mL
Type: Primary
Clonality: Polyclonal
Isotype: IgG
Conjugation: Unconjugated
Application: Western Blot, Immunofluorescence
Storage: For continuous use, store at 2-8 °C for one-two days. For extended storage, store in -20 °C freezer. Working dilution samples should be discarded if not used within 12 hours.
Special instructions: The antibody solution should be gently mixed before use.
Goat anti-V5 Polyclonal IgG Antibody
In the realm of proteomics and molecular biology, the Goat Anti-V5 Polyclonal IgG Antibody emerges as a critical tool, offering unparalleled specificity and reliability. This antibody targets the V5 epitope, a commonly used tag in the study of protein expression and function. Here, we explore the production, applications, and significance of this versatile antibody.
Understanding Goat Anti-V5 Polyclonal IgG Antibody
Production and Characteristics:
The Goat Anti-V5 Polyclonal IgG Antibody is produced by immunizing goats with a synthetic peptide that corresponds to the V5 epitope. This process leads to the generation of IgG antibodies capable of specifically recognizing the V5 tag. Polyclonal antibodies, such as this, are known for their ability to recognize multiple epitopes, potentially increasing the chances of detection.
Advantages of Goat-Derived Antibodies:
Goats, as a source of antibodies, offer several advantages. They typically produce a higher yield of antibodies than smaller animals and can generate robust responses to a wide range of antigens. This makes goat-derived antibodies particularly effective for diverse experimental needs.
Applications in Scientific Research
1. Protein Detection and Analysis:
The primary application of the Goat Anti-V5 Polyclonal IgG Antibody lies in detecting and analyzing proteins tagged with the V5 epitope. This is particularly valuable in studying protein localization, function, and protein-protein interactions.
2. Western Blotting:
In Western blotting, this antibody is used to detect V5-tagged proteins, ensuring accurate identification and quantification. Its high sensitivity makes it a go-to choice for researchers.
3. Immunoprecipitation (IP):
For immunoprecipitation, the antibody efficiently pulls down V5-tagged proteins from complex mixtures, facilitating the study of protein interactions and functions.
4. Immunofluorescence (IF):
In IF assays, the Goat Anti-V5 Polyclonal IgG Antibody allows for the visualization of V5-tagged proteins within cells, providing insights into protein distribution and localization.
5. Flow Cytometry:
This antibody is also instrumental in flow cytometry, enabling the detection and analysis of V5-tagged proteins in various cell populations.
Conclusion
The Goat Anti-V5 Polyclonal IgG Antibody is a potent tool in proteomics and molecular biology. Its specificity to the V5 epitope, coupled with the advantages of being goat-derived, makes it indispensable for researchers aiming to uncover the complexities of protein dynamics. As we delve deeper into the molecular foundations of life, tools like this antibody stand at the forefront, aiding in the critical analysis and understanding of proteins.