AffiVET® Poultry Newcastle Disease NDV Antigen Rapid Test Kit
Rapid chromatographic immunoassay for the qualitative detection of Newcastle Disease Virus antigen in chicken samples.
Product Name
AffiVET® Poultry Newcastle Disease NDV Antigen Rapid Test Kit
Catalog Number
AFG-VR-76
Package Size
10 Tests/Kit
Intended Use
The AffiGEN AffiVET Rapid NDV Ag Test Kit is a rapid chromatographic immunoassay for the qualitative detection of Newcastle Disease Virus antigen, also known as NDV Ag, in chicken samples. The test is intended as a rapid aid in the detection of NDV antigen.
| Sample Type | Use |
|---|---|
| Oropharyngeal swab | Recommended |
| Tracheal swab | Suitable |
| Spleen sample / homogenate | Suitable |
| Kidney sample / homogenate | Suitable |
Principle of the Test
The test uses NDV-specific antibodies as capture and detector antibodies. When NDV antigen is present in the sample, a visible test line, marked T, appears in the result window. The control line, marked C, confirms that the test has run correctly.
Kit Components
Each kit is supplied for 10 tests.
| Component | Quantity |
|---|---|
| AffiGEN AffiVET Rapid NDV Ag Test Device | 10 |
| Assay diluent tube | 10 |
| Disposable swab | 10 |
| Disposable dropper | 10 |
| Instructions for Use | 1 |
Materials Required but Not Provided
| Material | Purpose |
|---|---|
| Timer | Reading the result at the correct time |
| PBS | For spleen or kidney homogenate preparation, if required |
| Protective gloves | Safe sample handling |
Storage and Stability
- Store the kit at 2–30°C.
- Do not freeze.
- Do not expose to direct sunlight.
- Use the kit before the expiry date printed on the label.
Sample Collection and Preparation
Swab Samples
Collect an oropharyngeal or tracheal swab from the chicken. Use the swab immediately when possible.
Spleen or Kidney Samples
Prepare a 20–50% homogenate using PBS.
| Homogenate Preparation | Example |
|---|---|
| 50% homogenate | 2 g tissue + 2 mL PBS |
| 20% homogenate | 1 g tissue + 4 mL PBS |
Sample Storage
| Storage Condition | Maximum Duration |
|---|---|
| 2–8°C | Up to 24 hours |
| ≤ -20°C | Longer storage |
Allow frozen samples to reach room temperature before testing.
Test Procedure
Interpretation of Results
| Result | Appearance | Interpretation |
|---|---|---|
| Negative | C line only | NDV antigen not detected |
| Positive | C line + T line | NDV antigen detected |
| Invalid | No C line | Test invalid; repeat with a new device |
Performance Characteristics
| Parameter | Reported Value |
|---|---|
| Sensitivity vs RT-PCR | 94.7% |
| Specificity vs RT-PCR | 96.4% |
| Detection limit | Approx. 0.25 HAU |
Comparison with RT-PCR
| RT-PCR Positive | RT-PCR Negative | |
|---|---|---|
| AffiVET Rapid NDV Ag Positive | 18 | 3 |
| AffiVET Rapid NDV Ag Negative | 1 | 81 |
The product sheet also reports no cross-reaction with other avian viruses and states that the test detects all NDV.
Limitations
- A negative result does not fully exclude NDV infection, especially when antigen levels are below the detection limit or when sample quality is poor.
- Final diagnosis should not be based only on a single rapid test result. Clinical signs, flock history, epidemiological information, and additional laboratory testing should be considered when needed.
- False positive or false negative results may occur rarely.
Precautions
- Use only with chicken samples.
- Do not use expired kits.
- Do not use a test device if the foil pouch is damaged.
- Do not reuse test devices, swabs, droppers, or diluent tubes.
- Do not mix components from different lot numbers.
- Handle all samples as potentially infectious.
- Dispose of used materials according to local biosafety regulations.
Request Information or Quotation
Contact AffiGEN for availability, documentation, and commercial support for the AffiVET® Poultry Newcastle Disease NDV Antigen Rapid Test Kit.
Contact AffiGEN Visit Website