AffiPURE® Blood DNA Isolation Mini Kit V2
AffiPURE® Blood DNA Isolation Mini Kit V2 is specially designed for rapid and efficient purification of genomic DNA from 0.1 ml - 1 ml of fresh and frozen whole blood with common anticoagulants. The kit utilizes silica-based membrane technology in the form of a convenient spin column, eliminating the need for toxic phenol-chloroform extractions, or time-consuming ethanol precipitation. RNA, proteins, Lipids and other impurities such as enzyme inhibitors are removed effectively by the wash buffer. Isolated DNA with high purity and stable quality can be used directly in down-steam applications including restriction enzyme digestion, PCR, Southern blotting.
Components:
| Components |
AFG-VZ-DC111-01 50 Reactions |
AFG-VZ-DC111-02 200 Reactions |
| ACK Lysis Buffer | 100 mL | 2 x 200 mL |
| Proteinase K | 1 mL | 4 mL |
| Buffer BCL | 12 mL | 48 mL |
| Buffer WA | 15 mL | 60 mL |
| Buffer WB | 20 mL | 2 x 40 mL |
| Elution Buffer | 20 mL | 80 mL |
| FastPure gDNA Mini Columns | 50 Columns | 2 x 100 Columns |
| Collection Tubes 2 ml | 50 Columns | 2 x 100 Columns |
Note:
ACK Lysis Buffer: used for cracking red blood cells in blood samples;
Proteinase K: used for enzyme digestion of blood samples;
Buffer BCL: used for cells lysis and providing binding condition of the DNA to the membrane;
Buffer WA: used for the removal of protein residues;
Buffer WB: used for the removal of salt ions residues;
Elution Buffer: used for the elution of genomic DNA;
FastPure gDNA Mini Columns: used for adsorbing genomic DNA.
Collection Tubes 2 mL: collection tube for filtrate.
Storage:
All components except Proteinase K are stored and transported at room temperature (15°C - 25°C). Proteinase K can be stored at 15°C - 25°C for three months (at 2°C - 8°C for long-term), and transported at room temperature.
The buffer may precipitate when stored at low temperature. Dissolve at room temperature for a while or at 37°C for 10 min to thaw the precipitation and mix thoroughly before use.
Applications:
0.1 ml - 1 ml of fresh or frozen anticoagulated blood with non-nucleated red blood cells;5 µl - 20 µl of fresh or frozen anticoagulated blood with nucleated red blood cells.