AffiPURE® Blood/Cell/Tissue/Bacteria DNA Isolation Mini Kit

SKU: AFG-VZ-DC112-01

AffiPURE® Blood/Cell/Tissue/Bacteria DNA Isolation Mini Kit

AffiGEN
1 827 SEK
1 827 SEK
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AffiPURE® Blood/Cell/Tissue/Bacteria DNA Isolation Mini Kit

AffiPURE® Blood/Cell/Tissue/Bacteria DNA Isolation Mini Kit is suitable for extracting genomic DNA from ≤200 μL of fresh or frozen anticoagulated whole blood, <25 mg of animal tissues, <5 × 10^6 of cultured cells and <3 × 10^9 cultured bacterial samples. The kit is based on silica gel column purification technology that eliminates the need for extraction using phenol/chloroform organic solvents or time-consuming alcohol precipitation step. With this kit, RNA, proteins, lipids and other inhibitory impurities can be removed at the greatest extent. The DNA obtained can be directly used in PCR, qPCR, enzyme digestion and virus detection.

Components:

Components AFG-VZ-DC112-01
50 Reactions 		AFG-VZ-DC112-02
100 Reactions
Buffer ACL 12 mL 48 mL
Proteinase K 1 mL 4 mL
Buffer BCL 12 mL 48 mL
Buffer WA 15 mL 60 mL
Buffer WB 20 mL 2 x 40 mL
Elution Buffer 20 mL 80 mL
AffiPURE gDNA Mini Columns II 50 2 x 100
Collection Tubes 2 ml 50 2 x 100

 

Buffer ACL: Sample lysis.
Proteinase K: Perform enzymatic lysis of samples.
Buffer BCL: Perform lysis of samples and provide the binding environment.
Buffer WA: Remove residual protein contamination.
Buffer WB: Remove salt ions.
Elution Buffer: Elute the bound DNA.
AffiPURE gDNA Mini Columns II: Genomic DNA adsorption column.
Collection Tubes 2 mL: Filtrate collection tubes.

 

Storage:

Store at 15 ~ 25°C and transport at room temperature. If ambient temperatures often exceed 25°C, we suggest storing Proteinase K at 2 ~ 8°C.

Applications:

Fresh or frozen anticoagulated whole blood without nuclei (≤200 μl).
Fresh or frozen anticoagulated whole blood with nuclei (5 - 20 μl).
Cultured cells (<5 × 10^6 ).
Animal tissues (<25 mg).
Bacteria (<3 × 10^9 ).